Proteomics

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New nodes and edges in the glucosinolate molecular network revealed by proteomics and metabolomics of Arabidopsis myb28/29 and cyp79B2/B3


ABSTRACT: In this project we aimed to investigate new connections (edges) in the glucosinolate metabolism molecular network. We studied proteome changes in myb28/29 and cyp79B2/B3 Arabidopsis thaliana mutants as an effect of glucosinolate metabolism perturbation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Ning Zhu  

LAB HEAD: Sixue Chen

PROVIDER: PXD002284 | Pride | 2016-03-01

REPOSITORIES: Pride

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Publications

New nodes and edges in the glucosinolate molecular network revealed by proteomics and metabolomics of Arabidopsis myb28/29 and cyp79B2/B3 glucosinolate mutants.

Mostafa Islam I   Zhu Ning N   Yoo Mi-Jeong MJ   Balmant Kelly M KM   Misra Biswapriya B BB   Dufresne Craig C   Abou-Hashem Maged M   Chen Sixue S   El-Domiaty Maher M  

Journal of proteomics 20160223


Glucosinolates present in Brassicales are important for human health and plant defense against insects and pathogens. Here we investigate the proteomes and metabolomes of Arabidopsis myb28/29 and cyp79B2/B3 mutants deficient in aliphatic glucosinolates and indolic glucosinolates, respectively. Quantitative proteomics of the myb28/29 and cyp79B2/B3 mutants led to the identification of 2785 proteins, of which 142 proteins showed significant changes in the two mutants compared to wild type (WT). By  ...[more]

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