Dataset Information

Host response to S. pneumoniae TIGR4 and ΔpotABCD

ABSTRACT: The aim of this experiment is to determine the difference in protein expression, if any, in a mouse model of pneumococcal pneumonia using wild type and a polyamine transport operon deletion mutant strain of S. pneumoniae TIGR4, using expression proteomics. Eight week old female C57bl/6 mice were used in this study. The experimental design had two groups of 3 animals each that were administered 50 µL 1 X 107 CFU wild type S. pneumoniae TIGR4 or S. pneumoniae TIGR4 ΔpotABCD strain by intranasal instillation. The control group with three animals received 50 µL PBS. Mice were euthanized 4 hr and 12 hr post infection and lung tissues were harvested. The control group animals were euthanized 12 hr post infection.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Lung, Epithelial Cell

DISEASE(S): Bacterial Pneumonia

SUBMITTER: Joseph Reddy

LAB HEAD: Bindu Nanduri

PROVIDER: PXD002300 | Pride | 2018-10-27

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	AS_215021_EF30_1.raw	Raw
	AS_215021_EF30_1_MouseStrepContDb.msf	Msf
	AS_215021_EF30_1_MouseStrepContDb.msf-pride.pride.mgf.gz	Mgf
	AS_215021_EF30_2.raw	Raw
	AS_215021_EF30_2_MouseStrepContDb.msf	Msf

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Publications

Polyamine transporter in Streptococcus pneumoniae is essential for evading early innate immune responses in pneumococcal pneumonia.

Rai Aswathy N AN Thornton Justin A JA Stokes John J Sunesara Imran I Swiatlo Edwin E Nanduri Bindu B

Scientific reports 20160601

Streptococcus pneumoniae is the most common bacterial etiology of pneumococcal pneumonia in adults worldwide. Genomic plasticity, antibiotic resistance and extreme capsular antigenic variation complicates the design of effective therapeutic strategies. Polyamines are ubiquitous small cationic molecules necessary for full expression of pneumococcal virulence. Polyamine transport system is an attractive therapeutic target as it is highly conserved across pneumococcal serotypes. In this study, we c ...[more]

PMID: 27247105

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