Proteomics

Dataset Information

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Panorama of ancient metazoan macromolecular complexes: Dictyostelium discoideum


ABSTRACT: By combining extensive biochemical fractionation with quantitative mass spectrometry, we directly examined the composition of soluble multiprotein complexes among diverse animal models. The project has been jointly supervised by Andrew Emili and Edward M. Marcotte. Project website: http://metazoa.med.utoronto.ca

INSTRUMENT(S): LTQ Orbitrap Velos, LTQ XL

ORGANISM(S): Dictyostelium Discoideum Ax4 Dictyostelium Discoideum (slime Mold)

SUBMITTER: Blake borgeson  

LAB HEAD: Andrew Emili

PROVIDER: PXD002320 | Pride | 2015-06-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Dd.fasta Fasta
WAN111008_HCW_Ax4_wce_P1A01.MSGFDB_out Other
WAN111008_HCW_Ax4_wce_P1A01.inspect_out Other
WAN111008_HCW_Ax4_wce_P1A01.raw Raw
WAN111008_HCW_Ax4_wce_P1A01.results.pep.xml.xcorr_hit_list_best Pepxml
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Publications


Macromolecular complexes are essential to conserved biological processes, but their prevalence across animals is unclear. By combining extensive biochemical fractionation with quantitative mass spectrometry, here we directly examined the composition of soluble multiprotein complexes among diverse metazoan models. Using an integrative approach, we generated a draft conservation map consisting of more than one million putative high-confidence co-complex interactions for species with fully sequence  ...[more]

Publication: 1/2