Proteomics

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Changes in the ascending aorta proteome of cholesterol-fed rabbits

ABSTRACT: Here we undertook a proteomic investigation of ascending aorta from New Zealand White rabbits after 10 weeks on a high (2% w/w) cholesterol diet (HCD, n=5) or control diet (n=5) in order to profile the proteomic changes in response to the HCD. Histology confirmed intimal thickening in the HCD group and LC-MS/MS analysis of individually obtained ascending aorta extracts labelled with isobaric (iTRAQ) tags led to identification and quantitation of 453 unique proteins above the 1% false discovery rate threshold. Of 67 proteins showing significant differences in relative abundance (p<0.05), 62 were elevated and five decreased in ascending aorta from HCD-fed rabbits compared to controls. Six proteins were selected for validation using Multiple Reaction Monitoring which confirmed the iTRAQ results.

INSTRUMENT(S): QSTAR

ORGANISM(S): Oryctolagus Cuniculus (rabbit)

TISSUE(S): Heart

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Martin Middleditch  

LAB HEAD: Garth J.S. Cooper

PROVIDER: PXD002345 | Pride | 2018-06-21

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
12Mar13RabbitAorta1.7z Other
12Mar13RabbitAorta1.xml Xml
13-18Mar13RabbitAorta2.7z Other
13-18Mar13RabbitAorta2.xml Xml
RabbitAortaiTRAQRun112Mar13.7z Other
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Publications

Modelling atherosclerosis by proteomics: Molecular changes in the ascending aortas of cholesterol-fed rabbits.

Xu Jingshu J   Jüllig Mia M   Middleditch Martin J MJ   Cooper Garth J S GJ  

Atherosclerosis 20150710 1

The cholesterol-fed rabbit is commonly used as a model to study the vascular effects of hypercholesterolemia and resulting atherosclerotic lesions. Here we undertook a proteomic case-control investigation of ascending aortas from male New Zealand White rabbits after 10 weeks on a high-cholesterol (2% w/w) diet (HCD, n = 5) or control diet (n = 5), in order to determine the changes in response to the HCD. Histology confirmed intimal thickening in the HCD group consistent with atherosclerosis, and  ...[more]

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