Dataset Information

LC-MS/MS proteomic profiling of maize opaque endosperm mutants

ABSTRACT: Reduced prolamin (zein) accumulation and defective ER-body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and mucronate (Mc) whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. We used a LC-MS/MS proteomics approach to compare non-zein proteins of nearly isogenic opaque mutants. In total, 2762 proteins were identified that were enriched for biological processes such as protein transport and folding, amino acid biosynthesis, and proteolysis. Amino acid analysis of top proteins revealed qualitative changes in lysine abundance contributing to the overall lysine increase. Principal component analysis and pathway enrichment suggested that the mutants partition into three groups: Mc, DeB30, fl2 and o2; o1; and fl1. Indicator species analysis revealed mutant-specific proteins, and highlighted ER secretory pathway components that are enriched in selected groups of mutants, albeit with no common proteins across all mutants. Most significantly changed proteins were related to stress or defense and zein partitioning into the soluble fraction for Mc, DeB30, o1 and fl1 specifically. We suggest that ER stress is a universal trigger of opacity regardless of qualitative or quantitative changes in zein accumulation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Endosperm

SUBMITTER: Shangang Jia

LAB HEAD: David R. Holding

PROVIDER: PXD002379 | Pride | 2016-01-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	ProXML_withoutSorghum_20150616.xml	Xml
	qe2_01032014_10_WT3.raw	Raw
	qe2_01032014_15_o2_1_140106135019.raw	Raw
	qe2_01032014_17_o2_2_140106164728.raw	Raw
	qe2_01032014_19_o2_3_140106194519.raw	Raw

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Publications

Proteomic profiling of maize opaque endosperm mutants reveals selective accumulation of lysine-enriched proteins.

Morton Kyla J KJ Jia Shangang S Zhang Chi C Holding David R DR

Journal of experimental botany 20151227 5

Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein pr ...[more]

PMID: 26712829

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