Proteomics

Dataset Information

0

Daphnia pulex_Lysine acetylation


ABSTRACT: Daphnia (Daphnia pulex) is a small planktonic crustacean and a key constituent of aquatic ecosystems. It is commonly used as a model organism for studying environmental toxic challenges. In the past decade, a Daphnia genomic information and proteomic dataset has been developed. This dataset has expanded the opportunity to relate toxicological effects with “Daphnia proteomics” as it integrates proteomic knowledge in Daphnia, those approach will provide greater insights for toxicological research. In order to exploit Daphnia for ecotoxicological research, information on the post-translational modification (PTM) of proteins is necessary as this is a critical regulator of biological processes. Acetylation of lysine (Kac) is a reversible and highly regulated PTM that is associated with diverse biological functions. However, a comprehensive description of Kac in Daphnia is not yet available. Here, to understand the cellular distribution of lysine acetylation in Daphnia, we identified 98 acetylation sites in 65 proteins by immunoprecipitation using an anti-acetyllysine antibody and an liquid chromatography system supported by mass spectroscopy. We identified 28 acetylated sites connected with metabolic proteins and 6 acetylated enzymes associated with the TCA cycle in Daphnia. From GO and KEGG enrichment analyses, we showed that Kac in D. pulex is highly enriched in proteins associated with metabolic processes. Our data provide the first global analysis of lysine acetylation in D. pulex. The expanded proteomic dataset will be an important resource for the functional analysis of Kac in D. pulex and it will be nice to have a first step done using a promising future model organism.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Daphnia Pulex (water Flea)

SUBMITTER: Oh Kwang Kwon  

LAB HEAD: Sangkyu Lee

PROVIDER: PXD002441 | Pride | 2016-01-05

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
2014_Daphnia_Kac.raw Raw
2014_Daphnia_Kac.zip Other
uniprot-Daphnia_RefSeq_6669.fasta Fasta
Items per page:
1 - 3 of 3
altmetric image

Publications

Global proteomic analysis of protein acetylation affecting metabolic regulation in Daphnia pulex.

Kwon Oh Kwang OK   Sim Juhee J   Kim Sun Ju SJ   Oh Hye Ryeung HR   Nam Doo Hyun DH   Lee Sangkyu S  

Biochimie 20151215


Daphnia (Daphnia pulex) is a small planktonic crustacean and a key constituent of aquatic ecosystems. It is generally used as a model organism to study environmental toxic problems. In the past decade, genomic and proteomic datasets of Daphnia have been developed. The proteomic dataset allows for the investigation of toxicological effects in the context of "Daphnia proteomics," resulting in greater insights for toxicological research. To exploit Daphnia for ecotoxicological research, information  ...[more]

Similar Datasets

2014-10-31 | PXD001257 | Pride
2012-03-13 | E-GEOD-27959 | biostudies-arrayexpress
2016-09-12 | GSE80141 | GEO
2011-02-01 | E-GEOD-25823 | biostudies-arrayexpress
2012-03-13 | GSE27959 | GEO
2015-05-13 | E-GEOD-63275 | biostudies-arrayexpress
2015-05-13 | GSE63275 | GEO
2006-10-06 | GSE5908 | GEO
| PRJNA988494 | ENA
| PRJNA476561 | ENA