Dataset Information

Finding missing proteins from the epigenetically manipulated human cell with stringent quality criteria

ABSTRACT: There are three major epigenetic mechanisms, DNA methylation, histone modifications, and ncRNAs. The histone is a key player in epigenetics, and the acetylation and methylation are their most common post-translational modifications (PTMs). These histone modifications have important roles in transcriptional regulation, DNA repair, DNA replication, alternative splicing and chromosome condensation. For example, we previously found that H3.3 lysine 36 trimethylation (H3.3K36me3) histone and its reader protein BS69 could work together to regulate pre-mRNA process. Therefore, in this study, we established in vitro histone acetylation, demethylation and methylation models, respectively, by using human lung, liver and colorectal cancer cells.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Xinlei Lian

LAB HEAD: He Qing-Yu

PROVIDER: PXD002453 | Pride | 2022-03-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20150125_WQ_97H_1.wiff	Wiff
	20150125_WQ_97H_1.wiff.scan	Wiff
	20150125_WQ_97H_10.wiff	Wiff
	20150125_WQ_97H_10.wiff.scan	Wiff
	20150125_WQ_97H_2.wiff	Wiff

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Finding Missing Proteins from the Epigenetically Manipulated Human Cell with Stringent Quality Criteria.

Yang Lijuan L Lian Xinlei X Zhang Wanling W Guo Jie J Wang Qing Q Li Yaxing Y Chen Yang Y Yin Xingfeng X Yang Pengyuan P Lan Fei F He Qing-Yu QY Zhang Gong G Wang Tong T

Journal of proteome research 20150806 9

The chromosome-centric human proteome project (C-HPP) has made great progress of finding protein evidence (PE) for missing proteins (PE2-4 proteins defined by the neXtProt), which now becomes an increasingly challenging field. As a majority of samples tested in this field were from adult tissues/cells, the developmental stage specific or relevant proteins could be missed due to biological source availability. We posit that epigenetic interventions may help to partially bypass such a limitation b ...[more]

PMID: 26202631

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