Dataset Information

The Hepatocyte Proteome in Organotypic Rat Liver Models and the Influence of the Local Microenvironment

ABSTRACT: Liver models that closely mimic the in vivo microenvironment are central for understanding liver functions, capabilities, and intercellular communication processes. Whereas hepatocyte monolayers de-differentiate after only a few days in culture, constructs consisting of hepatocytes and non-parenchymal cells (NPC) separated by a polyelectrolyte multilayer (PEM) provide maintenance of hepatic phenotypes. To better understand the mechanisms and processes that underlie organotypic liver model function, hepatocyte protein abundances in the presence and absence of liver sinusoidal endothelial cells (LSECs) were compared to hepatocyte monolayers. The presence of the PEM led to increases in proteins associated with hepatocyte lipid metabolism, with mitochondrial-based β-oxidation and peroxisomal proteins found in higher abundance. It was also demonstrated that the presence of LSECs modulates levels of carboxylesterases and other phase I and phase II enzymes. These results are discussed in relation to intercellular signaling and hepatocyte function.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Hepatocyte, Liver

SUBMITTER: Lucas Vu

LAB HEAD: Richard F. Helm

PROVIDER: PXD002491 | Pride | 2017-07-06

REPOSITORIES: Pride

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			Action	DRS
	3DH1A_Pride.pride.mgf.gz	Mgf
	3DH1A_Pride.pride.mztab.gz	Mztab
	3DH1A_Pride.xml.gz	Xml
	3DH1B_Pride.pride.mgf.gz	Mgf
	3DH1B_Pride.pride.mztab.gz	Mztab

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The hepatocyte proteome in organotypic rat liver models and the influence of the local microenvironment.

Vu Lucas T LT Orbach Sophia M SM Ray W Keith WK Cassin Margaret E ME Rajagopalan Padmavathy P Helm Richard F RF

Proteome science 20160101

<h4>Background</h4>Liver models that closely mimic the in vivo microenvironment are useful for understanding liver functions, capabilities, and intercellular communication processes. Three-dimensional (3D) liver models assembled using hepatocytes and liver sinusoidal endothelial cells (LSECs) separated by a polyelectrolyte multilayer (PEM) provide a functional system while also permitting isolation of individual cell types for proteomic analyses.<h4>Methods</h4>To better understand the mechanism ...[more]

PMID: 28649179

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