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Intact Protein extraction using Liquid extraction surface analysis (LESA)

ABSTRACT: Development of a new technique of extraction of intact protein on localized region of tissue section using liquide extraction surface analysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Maxence Wisztorski

LAB HEAD: Michel Salzet

PROVIDER: PXD002514 | Pride | 2016-03-14

REPOSITORIES: Pride

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Publications

Spatially-resolved protein surface microsampling from tissue sections using liquid extraction surface analysis.

Wisztorski Maxence M Desmons Annie A Quanico Jusal J Fatou Benoit B Gimeno Jean-Pascal JP Franck Julien J Salzet Michel M Fournier Isabelle I

Proteomics 20160413 11-12

Tissue microenvironment characterization presents a challenge for a better understanding of the full complexity of a pathology. Unfortunately, making a precise "picture" of the disease needs an efficient microsampling method coupled to an accurate localization for performing region-dependent proteomics. Here, we present a method that enables rapid and reproducible extraction of proteins from a tissue section to analyze a specific region at a millimeter scale. The method used a liquid-microjuncti ...[more]

PMID: 26929135

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