Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Cell Culture, Cardiac Muscle Cell
SUBMITTER: Sophia Foehr
LAB HEAD: Jeroen Krijgsveld
PROVIDER: PXD002541 | Pride | 2016-08-17
REPOSITORIES: Pride
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Liao Yalin Y Castello Alfredo A Fischer Bernd B Leicht Stefan S Föehr Sophia S Frese Christian K CK Ragan Chikako C Kurscheid Sebastian S Pagler Eloisa E Yang Hao H Krijgsveld Jeroen J Hentze Matthias W MW Preiss Thomas T
Cell reports 20160721 5
RNA functions through the dynamic formation of complexes with RNA-binding proteins (RBPs) in all clades of life. We determined the RBP repertoire of beating cardiomyocytic HL-1 cells by jointly employing two in vivo proteomic methods, mRNA interactome capture and RBDmap. Together, these yielded 1,148 RBPs, 391 of which are shared with all other available mammalian RBP repertoires, while 393 are thus far unique to cardiomyocytes. RBDmap further identified 568 regions of RNA contact within 368 RBP ...[more]