Dataset Information

Translational regulation via ribosome modification

ABSTRACT: This dataset compares the proteomes of WT P. fluorescens and a delta-hfq mutant strain. This forms part of our characterisation of a new mechanism for translational control in bacteria, based on the covalent modification of a ribosomal protein by the enzyme RimK. RimK modification induces specific effects on ribosomal stability and function, which in turn affects the abundance of the important translational regulator Hfq. RimK is itself controlled by binding to the small regulatory proteins RimA and RimB and the widespread signalling molecule cyclic-di-GMP. Deletion of rimK compromises motility, virulence and plant colonisation/infection in several different Pseudomonas species. Changes in intracellular RimK activity enable Pseudomonas to translate environmental pressures into dynamic ribosomal changes, and in turn into adaptive phenotypic responses to the surroundings. This promotes motility and virulence during the initial stages of plant contact, and phenotypes including attachment, metabolite transport and stress control during long-term environmental adaptation.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Pseudomonas Fluorescens (strain Sbw25)

TISSUE(S): Cell Culture

SUBMITTER: Gerhard Saalbach

LAB HEAD: Dr Jacob George Malone

PROVIDER: PXD002573 | Pride | 2016-02-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	150501_01.CID.ITMS.sil0.150501_01.HCD.ITMS.sil0.mgf	Mgf
	150501_01.CID.ITMS.sil0.150501_01.HCD.ITMS.sil0.pride.mgf.gz	Mgf
	150501_01.raw	Raw
	150501_02.CID.ITMS.sil0.150501_02.HCD.ITMS.sil0.mgf	Mgf
	150501_02.CID.ITMS.sil0.150501_02.HCD.ITMS.sil0.pride.mgf.gz	Mgf

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Publications

Adaptive Remodeling of the Bacterial Proteome by Specific Ribosomal Modification Regulates Pseudomonas Infection and Niche Colonisation.

Little Richard H RH Grenga Lucia L Saalbach Gerhard G Howat Alexandra M AM Pfeilmeier Sebastian S Trampari Eleftheria E Malone Jacob G JG

PLoS genetics 20160204 2

Post-transcriptional control of protein abundance is a highly important, underexplored regulatory process by which organisms respond to their environments. Here we describe an important and previously unidentified regulatory pathway involving the ribosomal modification protein RimK, its regulator proteins RimA and RimB, and the widespread bacterial second messenger cyclic-di-GMP (cdG). Disruption of rimK affects motility and surface attachment in pathogenic and commensal Pseudomonas species, wit ...[more]

PMID: 26845436

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Project description:Pseudomonas fluorescens is implicated in food spoilage especially under cold storage, causing the increase of food waste and the reduction of environmental (and nutritional) sustainability. In food industry, Pseudomonas biofilm on pipe surfaces and facilities creates a persistent source of contamination and increases resistance to common disinfection strategies. Even though gene expression and protein pattern changes under environmental stimuli have been suggested, cellular mechanisms involved in biofilm production are poorly investigated for this species. In the present work, the cheese blue pigmenting P. fluorescens ITEM 17298 produced increased biofilm biomass and motility at 15 °C in comparison to 30 °C. Proteomic analysis revealed major differences of proteins involved in biofilm formation. In particular, at 15 °C we found increased amount of the c-di-GMP regulator PleD involved in motility as well as AlgB and the carbon storage regulator directly involved biofilm formation. Carbon metabolism, protein and fatty acid synthesis were also stimulated under cold incubation. Moreover, for the first time, enzymes correlated to indigoidine synthesis (blue pigment) were identified in the same condition. The differences in expression of regulatory proteins could therefore be the major determinant of the biofilm formation ability of P. fluorescens under refrigerated condition. Genomic and proteomic results also revealed virulence factors mostly detected at 30 °C. In order to counteract P. fluorescens persistence, this study also proposed a control strategy for biofilm development. Thus, a sub-lethal concentration of bovine lactoferrin hydrolysate (HLF) was assayed at both temperatures. Treatment caused a significant reduction in biofilm biomass, also confirmed by changes in proteome pattern. At each condition proteins involved regulatory functions and transcription factors were downregulated after HLF-treatment. Among these, at 15 °C we found that PleD was absent in treated samples, whilst negative regulators of alginate biosynthesis were detected; likewise, indigoidine related proteins were repressed. The cyclic-di-GMP-binding biofilm dispersal mediator was instead detected in all treated samples.