Proteomics

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Proteomic changes in liver of Channa striatus in response to high temperature stress


ABSTRACT: Climate change and resulting global warming are challenging environmental problems. Understanding the thermal adaptation mechanisms and survival strategies are of paramount importance. Fish has served as excellent animal model for understanding many biological processes. The present study was undertaken to investigate the proteomic changes in liver of murrel Channa striatus exposed to high temperature stress. Fishes were exposed to 36 °C for 4 days and liver proteome changes were analyzed using gel- based proteomics i.e. 2D gel electrophoresis, MALDI-TOF-MS and validation by transcript analysis. The study showed, besides others, up regulation of two sets of proteins, the antioxidant enzymes SOD, ferritin, GST and chaperones HSP60, PDI which was validated by transcript analysis. Further, gene expression analysis was also carried out in the fishes exposed to thermal stress for longer duration (30 days, in the laboratory and beyond, taking Channa collected from a hot spring runoff at a water temperature 36-38 °C); hsp60, sod and gst were found to continue to remain up regulated at 11, 8 and 3 folds, respectively in the hot spring runoff fish. Thus the study showed that SOD, GST and HSP60 play important role in thermal adaptation and survival under chronic heat stress.

INSTRUMENT(S): SCIEX instrument model

ORGANISM(S): Channa Striata (snakehead Murrel) (ophicephalus Striatus)

TISSUE(S): Hepatocyte, Liver

SUBMITTER: Bimal Mohanty  

LAB HEAD: Bimal Prasanna Mohanty

PROVIDER: PXD002608 | Pride | 2016-04-12

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
F168063_CHL15.mgf Mgf
F168063_CHL15.xml Xml
F168065_CHL11.mgf Mgf
F168065_CHL11.xml Xml
F168067_CHL12.mgf Mgf
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Publications

Proteomic changes in the liver of Channa striatus in response to high temperature stress.

Mahanty Arabinda A   Purohit Gopal Krishna GK   Banerjee Sudeshna S   Karunakaran Dhanasekar D   Mohanty Sasmita S   Mohanty Bimal Prasanna BP  

Electrophoresis 20160509 12


The present study was undertaken to investigate the proteomic changes in the liver of murrel Channa striatus exposed to high temperature stress. Fishes were exposed to 36°C for 4 days and liver proteome changes were analyzed using gel- based proteomics, i.e. 2DE, MALDI-TOF/TOF-MS, and validation by transcript analysis. The study showed, besides others, increased abundance of two sets of proteins, the antioxidative enzymes superoxide dismutase (SOD), ferritin, cellular retinol binding protein (CR  ...[more]

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