Proteomics

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In vivo protein-protein interaction in C. elegans early embryos

ABSTRACT: We combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Embryo

SUBMITTER: Jia-Xuan Chen  

LAB HEAD: Matthias Selbach

PROVIDER: PXD002624 | Pride | 2016-02-29

REPOSITORIES: Pride

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Publications

In Vivo Interaction Proteomics in Caenorhabditis elegans Embryos Provides New Insights into P Granule Dynamics.

Chen Jia-Xuan JX   Cipriani Patricia G PG   Mecenas Desirea D   Polanowska Jolanta J   Piano Fabio F   Gunsalus Kristin C KC   Selbach Matthias M  

Molecular & cellular proteomics : MCP 20160224 5

Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development. Our network reflects known biology and is highly  ...[more]

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