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Protein turnover during in vitro human vocal fold mucosa remodeling

ABSTRACT: We present here the first study evaluating the dynamic proteome changes in the remodeling process of decellularized tissue by reseeded cells using vocal fold mucosa as the model system.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Vocal Fold, Fibroblast

DISEASE(S): Disease Free

SUBMITTER: Qiyao Li

LAB HEAD: Lloyd M Smith

PROVIDER: PXD002734 | Pride | 2016-01-06

REPOSITORIES: Pride

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			Action	DRS
	131212_SILAC_1w1_1.raw	Raw
	131212_SILAC_1w1_2.raw	Raw
	131212_SILAC_1w2_1.raw	Raw
	131212_SILAC_1w2_2.raw	Raw
	131212_SILAC_1w3_1.raw	Raw

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Publications

Protein turnover during in vitro tissue engineering.

Li Qiyao Q Chang Zhen Z Oliveira Gisele G Xiong Maiyer M Smith Lloyd M LM Frey Brian L BL Welham Nathan V NV

Biomaterials 20151214

Repopulating acellular biological scaffolds with phenotypically appropriate cells is a promising approach for regenerating functional tissues and organs. Under this tissue engineering paradigm, reseeded cells are expected to remodel the scaffold by active protein synthesis and degradation; however, the rate and extent of this remodeling remain largely unknown. Here, we present a technique to measure dynamic proteome changes during in vitro remodeling of decellularized tissue by reseeded cells, u ...[more]

PMID: 26724458

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