Proteomics,Multiomics

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How C/EBPa creates an elite cell state for reprogramming to pluripotency

ABSTRACT: Mouse B cells, upon ectopic expression of the transcription factor C/EBPalpha for 18h, can be reprogrammed to induced pluripotent stem (iPS) cells with extremely high efficiency. To understand the molecular control of this phenomenon we performed a time course proteomic analysis by label-free protein quantification of proteins during B cell reprogramming to iPS cells.

OTHER RELATED OMICS DATASETS IN: PRJNA227547PRJNA198734PRJNA227776PRJNA227774

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): B Cell, Embryonic Stem Cell

SUBMITTER: Michael Wierer  

LAB HEAD: Matthias Mann

PROVIDER: PXD002769 | Pride | 2016-02-24

REPOSITORIES: Pride

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Publications

C/EBPα poises B cells for rapid reprogramming into induced pluripotent stem cells.

Di Stefano Bruno B   Sardina Jose Luis JL   van Oevelen Chris C   Collombet Samuel S   Kallin Eric M EM   Vicent Guillermo P GP   Lu Jun J   Thieffry Denis D   Beato Miguel M   Graf Thomas T  

Nature 20131215 7487

CCAAT/enhancer binding protein-α (C/EBPα) induces transdifferentiation of B cells into macrophages at high efficiencies and enhances reprogramming into induced pluripotent stem (iPS) cells when co-expressed with the transcription factors Oct4 (Pou5f1), Sox2, Klf4 and Myc (hereafter called OSKM). However, how C/EBPα accomplishes these effects is unclear. Here we find that in mouse primary B cells transient C/EBPα expression followed by OSKM activation induces a 100-fold increase in iPS cell repro  ...[more]

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