Proteomic analysis of Rhizoctonia solani

ABSTRACT: Rhizoctonia solani is an important root infecting pathogen of a range of food staples worldwide including wheat, rice, maize, soybean, potato and others. Conventional resistance breeding strategies are hindered by the absence of tractable genetic resistance in any crop host. Understanding the biology and pathogenicity mechanisms of this fungus is important for addressing these disease issues however, little is known about how R. solani causes disease. This study capitalises on recent genomic studies by applying mass spectrometry based proteomics to identify soluble, membrane-bound and culture filtrate proteins produced under wheat infection and vegetative growth conditions. Many of the proteins found in the culture filtrate had predicted functions relating to modification of the plant cell wall, a major activity required for pathogenesis on the plant host, including a number found only under infection conditions. Other infection related proteins included a high proportion of proteins with redox associated functions and many novel proteins without functional classification.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Rhizoctonia Solani Ag-8 Wac10335

SUBMITTER: Jonathan Anderson  

LAB HEAD: Karam Singh

PROVIDER: PXD002806 | Pride | 2016-01-28

REPOSITORIES: Pride