Dataset Information

Mouse organ specific spectral library and DIA analysis of the plasma proteome

ABSTRACT: The plasma proteome is highly dynamic and variable, composed of proteins derived from surrounding tissues and cells. To investigate the complex processes that contribute to the plasma proteome homeostasis we developed a mass spectrometry based proteomics strategy to infer the origin of proteins detected in murine plasma. The strategy relies on the construction of a comprehensive protein tissue atlas from cells and highly vascularized organs using shotgun mass spectrometry. The protein tissue atlas was transformed to a spectral library for highly reproducible quantification of tissue specific proteins directly in plasma using SWATH-like data-independent mass spectrometry analysis. We show that the method can determine drastic changes of tissue specific protein profiles in blood plasma from mouse animal models with sepsis. The strategy can be extended to several other species advancing our understanding of the complex processes that contribute to the plasma proteome homeostasis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spleen, Heart, Erythrocyte, Blood Platelet, Primary Cell, Blood Cell, Blood Plasma, Leukocyte, Liver, Lung, Neutrophil, Kidney

SUBMITTER: Simon Hauri

LAB HEAD: Johan Malmstrom

PROVIDER: PXD002896 | Pride | 2015-11-23

REPOSITORIES: pride

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Publications

Large-scale inference of protein tissue origin in gram-positive sepsis plasma using quantitative targeted proteomics.

Malmström Erik E Kilsgård Ola O Hauri Simon S Smeds Emanuel E Herwald Heiko H Malmström Lars L Malmström Johan J

Nature communications 20160106

The plasma proteome is highly dynamic and variable, composed of proteins derived from surrounding tissues and cells. To investigate the complex processes that control the composition of the plasma proteome, we developed a mass spectrometry-based proteomics strategy to infer the origin of proteins detected in murine plasma. The strategy relies on the construction of a comprehensive protein tissue atlas from cells and highly vascularized organs using shotgun mass spectrometry. The protein tissue a ...[more]

PMID: 26732734

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