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Mouse Seminal Vesicle Preparations C4PR_LIV

ABSTRACT: Ejaculates contain a diverse mixture of sperm and seminal fluid proteins, the combination of which is crucial to male reproductive success under competitive conditions. Males should therefore tailor the production of different ejaculate components according to their social environment, with particular sensitivity to cues of sperm competition risk (i.e. how likely it is that females will mate promiscuously). Here we test this hypothesis using an established vertebrate model system, the house mouse (Mus musculus domesticus), combining experimental data with a quantitative proteomics analysis of seminal fluid composition. Our study tests for the first time how both sperm and seminal fluid components of the ejaculate are tailored to the social environment.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Seminal Vesicle Fluid, Sperm

SUBMITTER: Dean Hammond

LAB HEAD: Paula Stockley

PROVIDER: PXD002900 | Pride | 2015-10-01

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	F003578.dat	Other
	F003578.mgf	Mgf
	RammSV_10_6484.mzML	Mzml
	RammSV_11_6492.mzML	Mzml
	RammSV_12_6522.mzML	Mzml

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Publications

Sperm competition risk drives plasticity in seminal fluid composition.

Ramm Steven A SA Edward Dominic A DA Claydon Amy J AJ Hammond Dean E DE Brownridge Philip P Hurst Jane L JL Beynon Robert J RJ Stockley Paula P

BMC biology 20151027

<h4>Background</h4>Ejaculates contain a diverse mixture of sperm and seminal fluid proteins, the combination of which is crucial to male reproductive success under competitive conditions. Males should therefore tailor the production of different ejaculate components according to their social environment, with particular sensitivity to cues of sperm competition risk (i.e. how likely it is that females will mate promiscuously). Here we test this hypothesis using an established vertebrate model sys ...[more]

PMID: 26507392

Similar Datasets

Social status and ejaculate investment in the house mouse c4pr_liv

Project description:Sperm competition theory predicts that males should tailor ejaculates according to their social status. Here we test this in a model vertebrate, the house mouse (Mus musculus domesticus), combining experimental data with a quantitative proteomics analysis of seminal fluid composition. Our analyses reveal that both sperm production and the relative production of proteins found in seminal fluid differ according to social dominance. Notably, whereas dominant males produce and ejaculate more sperm, subordinate males produce greater relative amounts of key proteins used in the formation of copulatory plugs. These findings have important implications for understanding the dynamics and outcome of sperm competition.

2021-09-09 | PXD019727 | Pride

BMP-inhibition in Drosophila secondary cells remodels the seminal proteome, and self and rival ejaculate functions

Project description:Seminal fluid proteins (SFPs) exert potent effects on male and female fitness. These rapidly evolving and molecularly diverse proteins derive from multiple male secretory cells and tissues. In Drosophila melanogaster most SFPs are produced in the accessory glands, which are composed of ~1000 fertility-enhancing ‘main cells’ and ~40, more functionally cryptic, ‘secondary cells’. Previous work shows that inhibition of BMP-signalling in secondary cells suppresses secretion, leading to a surprising uncoupling of normal female post-mating responses: refractoriness stimulation is impaired, but offspring production is not. Secondary cell secretions might therefore make a highly specific contribution to the seminal proteome and ejaculate function; alternatively, they might play a more global – but hitherto-undiscovered – role in regulating SFPs and their interconnected functions. Here, we present data that supports the latter model. We show that in addition to previously reported phenotypes, secondary cell-specific BMP-inhibition compromises sperm storage and increases female sperm use efficiency. Moreover, it alters sperm competition: second male sperm enter storage more slowly, ejaculates are ejected later, and first male paternity share improves. This result suggests a novel constraint on ejaculate evolution whereby female refractoriness and sperm competitiveness cannot be simultaneously maximised. Using quantitative proteomics, we reveal changes to the seminal proteome that surprisingly encompass alterations to main cell-derived proteins, indicating important cross-talk between classes of SFP-secreting cells. Our results demonstrate that ejaculate composition and function emerge from the integrated action of multiple secretory cell-types suggesting that modification to the cellular make-up of seminal fluid-producing tissues is an important factor in ejaculate evolution.

2020-05-26 | PXD015253 | Pride

Ejaculate samples for male infertility screening

Project description:The problem of the male reproductive system has both medical and social importance. It is highlighted that spermatozoa or seminal plasma are suitable samples for proteomic analysis of the male reproductive system. We suggested that the ejaculate could serve as one of the analytic specimens to determine fertility, which obtainable with relative ease and which contains many proteins. Moreover, the testis-specific proteins could form the basis of protein panel for detecting disorders associated with male infertility. The current study aimed to examine the ejaculate proteome compared to the spermatozoa and seminal plasma proteomes to determine a panel of proteins that can be used to assess sperm quality. Our results showed that the ejaculate represented a productive source for understanding the male reproductive physiology and could serve as a potential origin for the identification of novel protein biomarkers related to spermatozoa function in infertile. We believe that proteomic analysis based on testis-specific proteins of ejaculate along with spermatozoa (or even instead) can be useful for distinguishing the fertile, sub fertile, and infertile men.

2024-05-23 | PXD046848 | Pride

The protein and transcript profiles of human semen

Project description:Transcriptome of testes was examined for comparison of transcript abundance with that of sperm/seminal fluid (as sequenced in separate study)

2016-06-01 | GSE69434 | GEO

A single cell atlas of the mouse seminal vesicle

Project description:During mammalian reproduction, sperm are delivered to the female reproductive tract bathed in a complex medium known as seminal fluid, which plays key roles in signaling to the female reproductive tract and in nourishing sperm for their onwards journey. The majority of seminal fluid is produced by a somewhat understudied organ known as the seminal vesicle. Here, we report a single-cell RNA-Seq atlas of the mouse seminal vesicle, generated using tissues obtained from 23 mice of varying ages, exposed to a range of dietary challenges. We define the transcriptome of the secretory epithelial cells in this tissue, identifying a relative homogeneous population of the epithelial cells responsible for producing the majority of seminal fluid. We also define the immune cell populations – including large populations of macrophages, dendritic cells, T cells, and NKT cells – which have the potential to play roles in producing various immune mediators present in seminal plasma. Together, our data provide a resource for understanding the composition of an understudied reproductive tissue with potential implications for paternal control of offspring development and metabolism.

2024-05-16 | GSE267191 | GEO

Sperm modulate uterine immune parameters relevant to embryo implantation and reproductive success

Project description:Seminal fluid factors modulate the female immune response at conception to facilitate embryo implantation and reproductive success. Whether sperm affect this response has not been clear. We evaluated global gene expression by microarray in the mouse uterus after mating with intact or vasectomized males. Intact males induced greater changes in gene transcription, prominently affecting pro-inflammatory cytokine and immune regulatory genes, with TLR4 signaling identified as a top-ranked upstream driver. Recruitment of neutrophils and expansion of peripheral regulatory T cells were elevated by seminal fluid of intact males. In vitro, epididymal sperm induced IL6, CXCL2, and CSF3 in uterine epithelial cells of wild-type, but not Tlr4 null females. Collectively these experiments show that sperm assist in promoting female immune tolerance by eliciting uterine cytokine expression through TLR4-dependent signaling. The findings indicate a biological role for sperm beyond oocyte fertilization, in modulating immune mechanisms involved in female control of reproductive investment. We used microarrays to detail the global programme of gene expression in mice following exposure to different components of seminal fluid

2021-02-25 | GSE167485 | GEO

Expression data from porcine endometrial epithelial cells induced by seminal exosomes

Project description:Purpose: Seminal fluid (SF) is considered as a regulator on environment establishing in female reproductive tract of various mammal species without mechanism elucidated completely. This study aimed to identified the function of exosome isolated from SF on porcine endometrium. Methods: Total RNA profiles of porcine endometrial epithelial cells with seminal exosomes treatment (EXO.) or not (CTRL.) were generated by deep sequencing, in triplicate, using HiSeq 2500. Trimmed sequences were analyzed on the basis of the TopHat/Cufflinks pipeline based on the porcine genome and reference annotations obtained from Ensembl genome browser (https://www.ensembl.org). Results: 10916 transcripts, from which 70 genes were up-regulated and 114 genes were down-regulated with q-values less than 0.05 in EECs with seminal exosome treatment compared to those without exosome treatment. Conclusions: Through the gene ontology (GO) biological process and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis which revealed significantly enriched or decreased molecular functions, Our work demonstrated that exosomes derived from porcine seminal fluid induced endometrial immune and inflammatory response via CCL20 and AMCFII regulation specifically, which may facilitate sperm cells survival and activation, and resulting in appropriate environment required for fertilization and even implantation.

2017-10-26 | GSE106173 | GEO

Quantitation of microRNAs in seminal fluid from men with and without prostate cancer

Project description:The objective of this study was to determine whether prostate cancer-associated miRNAs are present in seminal fluid and, if so, evaluate their diagnostic potential. Small RNA sequencing was used to profile and compare miRNAs in the non-sperm cellular fraction of seminal fluid from men with biopsy-proven cancer (one pooled sample from 6 men) and men with elevated serum PSA but negative biopsy results (one pooled sample from 6 men).

2014-04-15 | E-GEOD-56686 | biostudies-arrayexpress

Proteomic diversification of spermatostyles among six species of whirligig beetles

Project description:Seminal fluid protein (SFP) composition is complex and commonly assumed to be rapidly divergent due to functional interactions with both sperm and the female reproductive tract (FRT), both of which evolve rapidly. In addition to sperm, seminal fluid may contain structures, such as mating plugs and spermatophores. Here, we investigate the evolutionary diversification of a lesser-known ejaculate structure: the spermatostyle, which has independently arisen in several families of beetles and true bugs. We characterized the spermatostyle proteome, in addition to spermatostyle and FRT morphology, in six species of whirligig beetles (family Gyrinidae). Spermatostyles were enriched for proteolytic enzymes, and assays confirmed they possess proteolytic activity. Sperm leucyl aminopeptidases (S-LAPs) were particularly abundant, and their localization to spermatostyles was confirmed by immunohistochemistry. Although there was evidence for functional conservation of spermatostyle proteomes across species, phylogenetic regressions suggest evolutionary covariation between protein composition and the morphology of both spermatostyles and FRTs. We postulate that S-LAPs (and other proteases) have evolved a novel structural role in spermatostyles and discuss spermatostyles as adaptations for delivering male-derived materials to females.

2024-08-09 | PXD048928 | Pride

The protein and transcript profiles of human semen

Project description:Transcriptome of testes was examined for comparison of transcript abundance with that of sperm/seminal fluid (as sequenced in separate study) Commercially available (Ambion) human testes RNA was prepared and sequenced in two replicates

2016-06-01 | E-GEOD-69434 | biostudies-arrayexpress

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