Dataset Information

Cacao seed development - System level analysis of cacao seed ripening reveals a sequential interplay of primary and secondary metabolism leading to polyphenol accumulation and preparation of stress resistance

ABSTRACT: integration of metabolomics and proteomics for understanding the molecular physiology of cacao seed development. Both the metabolomic and proteomic profiles of 4 developing stages' cacao seed were obtained from the mass spectrometry based platforms.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Theobroma Cacao (cacao) (cocoa)

TISSUE(S): Seed

SUBMITTER: Lei Wang

LAB HEAD: Wolfram Weckwerth

PROVIDER: PXD002922 | Pride | 2016-05-09

REPOSITORIES: Pride

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System level analysis of cacao seed ripening reveals a sequential interplay of primary and secondary metabolism leading to polyphenol accumulation and preparation of stress resistance.

Wang Lei L Nägele Thomas T Doerfler Hannes H Fragner Lena L Chaturvedi Palak P Nukarinen Ella E Bellaire Anke A Huber Werner W Weiszmann Jakob J Engelmeier Doris D Ramsak Ziva Z Gruden Kristina K Weckwerth Wolfram W

The Plant journal : for cell and molecular biology 20160805 3

Theobroma cacao and its popular product, chocolate, are attracting attention due to potential health benefits including antioxidative effects by polyphenols, anti-depressant effects by high serotonin levels, inhibition of platelet aggregation and prevention of obesity-dependent insulin resistance. The development of cacao seeds during fruit ripening is the most crucial process for the accumulation of these compounds. In this study, we analyzed the primary and the secondary metabolome as well as ...[more]

PMID: 27136060

Similar Datasets

Project description:Plant embryos can survive years in a desiccated, quiescent state within seeds. In many species, seeds are dormant and unable to germinate at maturity. They acquire the capacity to germinate through a period of dry storage called after-ripening (AR), a biological process that occurs at 5-15% moisture when most metabolic processes cease. Because stored transcripts will be among the first proteins translated upon water uptake, they likely impact germination potential. Transcriptome changes associated with the increased seed dormancy of the GA-insensitive sly1-2 mutant, and with dormancy loss through sly1-2 after-ripening or constitutive overexpression of the GA receptor (GID1b) were characterized in dry seeds. This experiment used the same seed batches as a previous experiment (E-MTAB-4782) to characterize transcriptional changes associated with the increased seed dormancy and dormancy loss in imbibing seeds. The SLY1 gene encodes the F-box subunit of an SCF E3 ubiquitin ligase needed for GA-triggered proteolysis of DELLA repressors of seed germination. In the sly1-2 mutant, GA-directed DELLA proteolysis cannot occur leading to DELLA protein accumulation and increased dormancy. sly1-2 mutant seeds are fully dormant at 2 weeks of dry storage (0% germination), but germinate well with very long after-ripening (51% germination after 19 months). sly1-2 seed germination can also be rescued by overexpression of the GA receptor, GA-INSENSITIVE DWARF1b (GID1b-OE), which resulted in 74% germination at 2 weeks of dry storage. In this experiment, we sampled dry seeds of wild-type Ler at 2 weeks of dry storage (non-dormant), dormant sly1-2 (2 weeks of dry storage; sly1-2(D)), long after-ripened sly1-2 (non-dormant, 19 months of dry storage; sly1-2(AR)), and sly1-2 GID1b-OE (non-dormant, 2 weeks of dry storage). This experimental design allowed comparison between these transcriptomes in dry seeds to determine if dry seed stored mRNA differences contribute to the dormancy phenotypes observed once seeds are imbibed. Seeds for Ler wt, sly1-2(D), and sly1-2 GID1b-OE were grown alongside each other under the same conditions and after-ripened for 2 weeks. Seeds from sly1-2(AR) were grown under the same conditions in advance of the other lines to allow for the long after-ripening requirement. RNA was extracted using a phenol-chloroform-based extraction from three biological replicates per treatment.

			Action	DRS
	Cacao_Stage1_R1.RAW	Raw
	Cacao_Stage1_R2.RAW	Raw
	Cacao_Stage1_R3.RAW	Raw
	Cacao_Stage2_R1.RAW	Raw
	Cacao_Stage2_R2.RAW	Raw