Dataset Information

Phosphoprotein network analysis of white adipose tissues unveils deregulated pathways in response to high-fat diet

ABSTRACT: Despite wide efforts in the last decade, signaling aberrations associated with obesity remain enigmatic. Here, we carried out phosphoproteomic analysis of mouse white adipose tissues (WAT) upon low-fat diet (LFD) and high-fat diet (HFD) to dissect underlying molecular mechanisms of obesity. Of the 7696 phosphopeptides quantified, 191 proteins including various insulin-responsive proteins and metabolic enzymes functioning in lipid homeostasis, exhibited differential phosphorylation with high-fat feeding. Kinase predictions and integrated network analysis identified several deregulated kinase signaling pathways, and suggested possibilities of HFD-induced transcriptional rewiring. Further, functional validation of a novel HFD-responsive site on cytoplasmic acetyl-coA forming ACSS2 (S263) suggested that the phosphorylation is important in regulating insulin signaling and maintaining triglyceride levels. This study represents one of the first comprehensive phosphoproteome data in mouse obesity models, and describes a systems-level approach for identifying deregulated molecular events and potential therapeutic targets in the context of high-fat feeding and adipocyte perturbation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): White Adipose Tissue

SUBMITTER: Hyungwon Choi

LAB HEAD: Jayantha Gunaratne

PROVIDER: PXD002927 | Pride | 2016-05-20

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	QE_140428_22_IS095_Phosphoproteome.RAW	Raw
	QE_140428_22_IS095_Phosphoproteome.XML	Xml
	QE_140428_28_IS096_Phosphoproteome.RAW	Raw
	QE_140428_28_IS096_Phosphoproteome.XML	Xml
	QE_140428_37_IS097_Phosphoproteome.RAW	Raw

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Phosphoprotein network analysis of white adipose tissues unveils deregulated pathways in response to high-fat diet.

Shaik Asfa Alli AA Qiu Beiying B Wee Sheena S Choi Hyungwon H Gunaratne Jayantha J Tergaonkar Vinay V

Scientific reports 20160516

Despite efforts in the last decade, signaling aberrations associated with obesity remain poorly understood. To dissect molecular mechanisms that define this complex metabolic disorder, we carried out global phosphoproteomic analysis of white adipose tissue (WAT) from mice fed on low-fat diet (LFD) and high-fat diet (HFD). We quantified phosphorylation levels on 7696 peptides, and found significant differential phosphorylation levels in 282 phosphosites from 191 proteins, including various insuli ...[more]

PMID: 27180971

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