Dataset Information

The E. coli type III secreted effectors NleB and EspL interact with the microtubule associated protein ensconsin to disrupt vesicle trafficking, part 1

ABSTRACT: Enteropathogenic and enterohaemorrhagic E. coli (EPEC and EHEC) translocate a set of type III effector proteins into host cells that are critical for bacterial virulence. These effectors subvert normal host pathways by interacting with a variety of targets within the cell, but the binding partners and mechanism of action of the majority of effectors are not understood. We identified the microtubule associated protein, ensconsin, as a novel target for two EPEC/EHEC effectors. We found that the secreted effectors NleB and EspL bind host ensconsin and work synergistically to paralyze kinesin-based intracellular vesicular transport. Our findings demonstrate that EPEC/EHEC encode multiple effectors that control intracellular vesicle movement and suggest a simple strategy for broad-based immobilization of host cells by pathogens.

INSTRUMENT(S): LTQ FT, LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

SUBMITTER: Leonard Foster

LAB HEAD: Leonard James Foster

PROVIDER: PXD002933 | Pride | 2016-12-22

REPOSITORIES: Pride

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Publications

Quantitative Mass Spectrometry Identifies Novel Host Binding Partners for Pathogenic Escherichia coli Type III Secretion System Effectors.

Law Robyn J RJ Law Hong T HT Scurll Joshua M JM Scholz Roland R Santos Andrew S AS Shames Stephanie R SR Deng Wanyin W Croxen Matthew A MA Li Yuling Y de Hoog Carmen L CL van der Heijden Joris J Foster Leonard J LJ Guttman Julian A JA Finlay B Brett BB

Journal of proteome research 20160328 5

Enteropathogenic and enterohemorrhagic Escherichia coli cause enteric diseases resulting in significant morbidity and mortality worldwide. These pathogens remain extracellular and translocate a set of type III secreted effector proteins into host cells to promote bacterial virulence. Effectors manipulate host cell pathways to facilitate infection by interacting with a variety of host targets, yet the binding partners and mechanism of action of many effectors remain elusive. We performed a mass s ...[more]

PMID: 27018634

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			Action	DRS
	MSQuant.xls	Xls
	MascotOutputs.zip	Other
	NS_Sa04_060607.RAW	Raw
	NS_Sa05_060630.RAW	Raw
	NS_Sa05_060702.RAW	Raw