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Lineage specific metabolic activity in haematopoiesis

ABSTRACT: Proteins were relatively quantified from the isolated mitochondria undergoing differentiation along two distinct lineages.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Hematopoietic Stem Cell

SUBMITTER: Michael Walker

LAB HEAD: Anthony Whetton

PROVIDER: PXD002968 | Pride | 2017-09-07

REPOSITORIES: Pride

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			Action	DRS
	102212_claudia2_25.raw	Raw
	102212_claudia2_26.raw	Raw
	102212_claudia2_27.raw	Raw
	102212_claudia2_28.raw	Raw
	102212_claudia2_29.raw	Raw

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Publications

Features of lineage-specific hematopoietic metabolism revealed by mitochondrial proteomics.

Billing Claudia C Walker Michael M Noack Nicole N Böhme Christian C Ceglarek Uta U Niederwieser Dietger D Whetton Anthony A Cross Michael M

Proteomics 20170726 15-16

Hematopoietic bone marrow is a regenerative tissue of high clinical relevance, yet relatively little is known about the metabolism of the stem and progenitor populations concerned. We have used a multipotent murine cell line to generate sufficient numbers of cells undergoing self-renewal, erythroid or myeloid differentiation to allow a proteomics analysis of enriched mitochondria. Stringent analysis identified 37 mitochondria-associated proteins changing on differentiation in this system. Those ...[more]

PMID: 28665046

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Mammary epithelial cells have lineage-rooted metabolic identities

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Neural Lineage Progression Controlled by a Temporal Proliferation Program along the Apical-basial Axis

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Lineage-Specific Proteomic Signatures in the Mycobacterium Tuberculosis Complex Reveal Differential Abundance of Proteins Involved in Virulence, DNA Repair, CRISPR-Cas, Bioenergetics and Lipid Metabolism

Project description:Despite the discovery of Mycobacterium tuberculosis (Mtb) more than 130 years ago, Mtb physiology and the mechanisms of virulence are still not fully understood. The objective of this study was to compare and characterize the differentially abundant protein profiles of modern, pre-modern and ancient Mtb lineages. Using a comprehensive analysis of the proteome of Mtb lineages 3, 4, 5 and 7, unique and shared proteomic signatures in these modern, pre-modern and ancient Mtb lineages were delineated. Main proteomic findings were verified by using immunoblotting. In addition, analysis of multiple genome alignment of all lineages was performed using Geneious Prime software. Label-free peptide quantification of whole cells from Mtb lineage 3, 4, 5 and 7 yielded 38,346 unique peptides derived from 3092 proteins, representing 77% coverage of the predicted Mtb proteome. Mtb lineage-specific differential abundances was observed for 539 proteins. Lineage 7 exhibited a markedly reduced abundance of proteins involved in DNA repair, type VII ESX-3 and ESX-1 secretion systems, lipid metabolism and inorganic phosphate uptake, and an increased abundance of proteins involved in alternative pathways of the TCA cycle and the CRISPR/Cas system as compared to the other lineages. Lineages 3 and 4 exhibited a higher abundance of proteins involved in virulence, DNA repair, drug resistance and other metabolic pathways. The high throughput analysis of Mtb proteome by super resolution massspectrometry provided an insight into the differential expression of proteins between Mtb lineages 3, 4, 5 and 7 that may explain the slow growth and reduced virulence of lineage 7, as well as metabolic flexibility and the ability to survive under adverse growth conditions.

2020-09-01 | PXD020383 | Pride

Epithelial-Mesenchymal Micro-Niches Govern Stem Cell Lineage Choices (ATAC-seq)

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Gene expression profile at single cell level from the liver of sham and sepsis mice

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Mammary epithelial cells have lineage-rooted metabolic identities

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Mtg16 regulates E protein activity and lineage specification in dendritic cell development (ChIP-seq)

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2014-06-13 | E-GEOD-43876 | biostudies-arrayexpress

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