Dataset Information

Proteome analysis reveals alterations in protein profile upon ectomycorrhiza establishment in oak roots

ABSTRACT: Trees establish a symbiotic relationship with specialized soil fungi, called ectomycorrhizae, which is essential for nutrition, growth and health of temperate forest ecosystems. Understanding the mechanisms governing the establishment and functioning of ectomycorrhiza is important because of the role of forests in sequestering CO2 and also to develop ways to optimize tree productivity and sustainability. Here, we investigated the response of an oak species to ectomycorrhiza formation using a two dimensional differential in gel electrophoresis (2D-DIGE) and MALDI-TOF/TOF mass spectrometry proteomics approach. At the root level, changes in the abundance of 34 unique oak proteins were detected and revealed proteins involved in carbon and energy metabolism, protein processing and degradation, response to oxidative stress, lipid metabolism/transport, nitrogen and phosphorous assimilation and cell wall modification. Proteins supporting the importance of the secretory pathway functioning, in particular of the endoplasmic reticulum, during ectomycorrhiza functioning were identified. These proteins were identified as components of the endoplasmic reticulum folding/chaperoning machinery and proteins involved in the ER quality control system. This study constitutes an important contribution for the understanding of the mechanisms underlying the response of plants to ectomycorrhizal symbiosis establishment.

INSTRUMENT(S): 4800 Proteomics Analyzer

ORGANISM(S): Quercus

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Joana Martins

LAB HEAD: Ana Varela Coelho

PROVIDER: PXD003009 | Pride | 2017-07-14

REPOSITORIES: pride

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Publications

Oak protein profile alterations upon root colonization by an ectomycorrhizal fungus.

Sebastiana Mónica M Martins Joana J Figueiredo Andreia A Monteiro Filipa F Sardans Jordi J Peñuelas Josep J Silva Anabela A Roepstorff Peter P Pais Maria Salomé MS Coelho Ana Varela AV

Mycorrhiza 20161007 2

An increased knowledge on the real impacts of ectomycorrhizal symbiosis in forest species is needed to optimize forest sustainable productivity and thus to improve forest services and their capacity to act as carbon sinks. In this study, we investigated the response of an oak species to ectomycorrhizae formation using a proteomics approach complemented by biochemical analysis of carbohydrate levels. Comparative proteome analysis between mycorrhizal and nonmycorrhizal cork oak plants revealed no ...[more]

PMID: 27714470

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