Dataset Information

Comprehensive proteome profiling of nuclear and cytoplasmic fractions of rat Sertoli cells.

ABSTRACT: The Sertoli cells (Sc) of 5 days (infant) and 12 days (pubertal) old rat were isolated and cultured in triplicates. Nuclear and cytoplasmic fractionation was done for both the cases. All the four protein fractions (nuclear and cytoplasm of infant and pubertal Sc) were anaysed using Lc-MS/MS.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Sertoli Cell, Cell Culture

SUBMITTER: Kamal Mandal

LAB HEAD: Dr. Subeer S. Majumdar

PROVIDER: PXD003050 | Pride | 2017-01-18

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	12_day_cytoplasm.pep.xml	Pepxml
	12_day_cytoplasm_replicate_1.mzML	Mzml
	12_day_cytoplasm_replicate_2.mzML	Mzml
	12_day_cytoplasm_replicate_3.mzML	Mzml
	12_day_nucleus.pep.xml	Pepxml

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Publications

An integrated transcriptomics-guided genome-wide promoter analysis and next-generation proteomics approach to mine factor(s) regulating cellular differentiation.

Mandal Kamal K Bader Samuel L SL Kumar Pankaj P Malakar Dipankar D Campbell David S DS Pradhan Bhola Shankar BS Sarkar Rajesh K RK Wadhwa Neerja N Sensharma Souvik S Jain Vaibhav V Moritz Robert L RL Majumdar Subeer S SS

DNA research : an international journal for rapid publication of reports on genes and genomes 20170401 2

Differential next-generation-omics approaches aid in the visualization of biological processes and pave the way for divulging important events and/or interactions leading to a functional output at cellular or systems level. To this end, we undertook an integrated Nextgen transcriptomics and proteomics approach to divulge differential gene expression of infant and pubertal rat Sertoli cells (Sc).Unlike, pubertal Sc, infant Sc are immature and fail to support spermatogenesis. We found exclusive as ...[more]

PMID: 28065881

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Project description:Immune privileged Sertoli cells (SC) survive when transplanted across immunological barriers and prolong the survival of co-transplanted allogeneic and xenogeneic cells in rodent models. However, the mechanism for this survival and protection remains unresolved. We have recently identified a mouse Sertoli cell line (MSC-1) that lacks some of the immunoprotective abilities associated with primary SC. The objective of this study was to compare the survival and gene expression profiles of primary SC and MSC-1 cells to identify factors or immune-related pathways potentially important for SC immune privilege. Primary SC or MSC-1 cells were transplanted as allografts to the renal subcapsular area of naïve BALB/c mice and cell survival was analyzed by immunohistochemistry. Additionally, transcriptome differences were investigated by microarray and pathway analyses. While primary SC were detected within the grafts with 100% graft survival throughout the 20-day study, MSC-1 cells w ere rejected between 11 and 14 days with 0% graft survival at 20 days post-transplantation. Microarray analysis identified 3198 genes that were differentially expressed with a ± 4-fold or higher level in primary SC. Cluster and pathway analyses indicate that the mechanism of SC immune privilege is likely complex with multiple immune modulators being involved such as immunosuppressive cytokines and complement inhibitors, lipid mediators for controlling inflammation, and junctional molecules that control leukocyte movement in and out of the immune privileged space. Further study of these immune modulators will increase our understanding of SC immune privilege and in the long-term lead to improvements in transplantation success. We conducted microarray and pathway analyses to identify genes and immune-related pathways differentially regulated by aggregated primary Sertoli cells and MSC-1 cell line. Aggregated 19 to 20-day mice primary Sertoli cells and MSC-1 cell line were used to determine the global transcriptome differences important for the survival and protection of transplanted cells.