Dataset Information

Phosphoproteomics analysis of anti-cancer effects of ginsenoside Rg3 in human breast cancer cells

ABSTRACT: The breast cancer incidence has been increasing in China, with the earlier age of onset compared with Western countries. Traditional Chinese medicine has been provided as one of the major source of anti-cancer drugs. Ginseng is one of the most common traditional medicines in China. Ginsenosides, the saponins in the plant Panax (ginseng) are the major active components responsible for their chemopreventive effects from cancer. However, the mechanisms by which ginsenosides exert their anti-cancer effect remain elusive. By combining TMT-based quantitation with TiO2-based phosphopeptide enrichment, we performed a quantitative analysis of the changes of the phosphoproteomes in ginsenoside Rg3-treated breast cancer MDA-MB-231 cells. We were able to quantitate 5,140 phosphorylation sites on 2,041 phosphoproteins. Our data show that the phosphorylation status of 13 sites was changed in MDA-MB-231 cells upon Rg3 treatment. The perturbed phosphoproteins are CPSF7, EEF2, HIRIP3, MAGED2, MPRIP, MYCBP2, PAWR, PPP1R12A, RANBP2, SEPT9, TMPO, and UFL1. These proteins are involved in various biological processes, including protein synthesis, cell division, and inhibition of NF-κB signaling. Our study revealed that Rg3 exerts its anti-cancer effects through a combination of different signaling pathways.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Mammary Gland Epithelial Cell

DISEASE(S): Breast Cancer

SUBMITTER: Yi Fang

LAB HEAD: Hui Han

PROVIDER: PXD003092 | Pride | 2018-01-08

REPOSITORIES: Pride

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			Action	DRS
	GinsenosideRg3_Proteome_1.raw	Raw
	GinsenosideRg3_Proteome_2.raw	Raw
	GinsenosideRg3_Proteome_3.raw	Raw
	GinsenosideRg3_Proteome_4.raw	Raw
	GinsenosideRg3_Proteome_5.raw	Raw

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Publications

Phosphoproteomic analysis of the antitumor effects of ginsenoside Rg3 in human breast cancer cells.

Zou Mingjin M Wang Jing J Gao Jidong J Han Hui H Fang Yi Y

Oncology letters 20171219 3

The incidence of breast cancer has been increasing in China and the age of breast cancer onset is earlier compared with Western countries. Compounds commonly used in Traditional Chinese Medicine (TCM) are an important source of anticancer drugs. Ginseng is one of the most common medicines used in TCM. Ginsenosides, which are saponins found in the ginseng plant, are the major active components responsible for the chemopreventive effects of ginseng in cancer. However, the mechanisms by which ginse ...[more]

PMID: 29435015

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