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Bovine milk proteome during involution

ABSTRACT: This project tested the hypothesis that drying off and involution is accompanied by similar changes among the low abundance proteins as mastitis, thereby presenting a possible alternative means of maximising yields of host-defence-associated bioactive proteins.

INSTRUMENT(S): QSTAR

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Milk

SUBMITTER: Kasper Hettinga

LAB HEAD: Thomas T. Wheeler

PROVIDER: PXD003110 | Pride | 2016-06-14

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	GeLCMS_Bands31-35.wiff	Wiff
	GeLCMS_Bands36-40.wiff	Wiff
	GeLCMS_Bands41-60.wiff	Wiff
	GeLC_Skim_d0.mgf	Mgf
	GeLC_Skim_d3.mgf	Mgf

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1 - 5 of 256

Publications

Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution.

Boggs Irina I Hine Brad B Smolenksi Grant G Hettinga Kasper K Zhang Lina L Wheeler Thomas T TT

Data in brief 20160513

Here we provide data from three proteomics techniques; two-dimensional electrophoresis (2-DE) followed by identification of selected spots using PSD MALDI-TOF MS/MS, one-dimensional gel electrophoresis followed by LC-MS/MS analysis of gel slices (GeLC) and dimethyl isotopic labelling of tryptic peptides followed by Orbitrap MS/MS (DML), to quantify the changes in the repertoire of bovine milk proteins that occurs after drying off. We analysed skim milk and whey sampled at day 0 and either day 3 ...[more]

PMID: 27274532

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Bovine milk LCMSMS proteomics - Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution

Project description:This project tested the hypothesis that drying off and involution is accompanied by similar changes among the low abundance proteins as mastitis, thereby presenting a possible alternative means of maximising yields of host-defence-associated bioactive proteins

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Proteome compendium of the Antarctic krill (Euphausia superba) - Whole body

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RRBS (Reduced Representation Bisulfite Sequencing) of HL60

Project description:We exploited the methylation genome-scale screening RRBS to correlate the RNA species physically associated with DNMT1 and proximal to the annotated genes to the methylation status of the corresponding loci. Out of 15275 non ambiguous gene loci identified by DNMT1 RIP-Seq, 9436 loci were covered by RRBS. These 9436 loci were clustered according to the fold of specific DNMT1 library peaks enrichment (defined as the ratio of the sum of the area under the curve of specific DNMT1 library peaks covering the gene loci). Genes were then stratified by the expression profile ultimately leading to the epitranscriptome map, a comprehensive map cross-referencing DNMT1-interacting transcripts to (i) DNA methylation and (ii) gene expression profile. Relationship between DNMT1-RNA interactions, DNA methylation and gene expression

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