Dataset Information

Proteomic investigation of a mixed microbial culture synthesizing polyhydroxyalkanoates from fermented dairy manure under aerobic feast-famine conditions

ABSTRACT: Polyhydroxyalkanoates (PHAs) are bio-based, biodegradable polyesters that can be produced from organic-rich waste streams using mixed microbial cultures. To maximize PHA production, mixed microbial cultures may be enriched for PHA-producing bacteria with a high storage capacity through the imposition of cyclic, aerobic feast-famine conditions in a sequencing batch reactor (SBR). Though enrichment SBRs have been extensively investigated a bulk solutions-level, little evidence at the proteome level is available to describe the observed SBR behavior to guide future SBR optimization strategies. As such, the purpose of this investigation was to characterize proteome dynamics of a mixed microbial culture in an SBR operated under aerobic feast-famine conditions using fermented dairy manure as the feedstock for PHA production. At the beginning of the SBR cycle, excess PHA precursors were provided to the mixed microbial culture (i.e., feast), after which followed a long duration devoid of exogenous substrate (i.e., famine). Two-dimensional electrophoresis was used to separate protein mixtures during a complete SBR cycle, and proteins of interest were identified.

INSTRUMENT(S): Q-Tof Premier

ORGANISM(S): Environmental Samples

TISSUE(S): Prokaryotic Cell

SUBMITTER: Andrea Hanson

LAB HEAD: Erik Coats

PROVIDER: PXD003126 | Pride | 2017-01-31

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	1196AndreaHanson_061515_44.mzid.gz	Mzid
	1196AndreaHanson_061515_44.pkl	Other
	1196AndreaHanson_061515_44.pride.mgf.gz	Mgf
	1196AndreaHanson_061515_44.pride.mztab.gz	Mztab
	1196AndreaHanson_061515_44.raw.zip	Raw

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Publications

Community proteomics provides functional insight into polyhydroxyalkanoate production by a mixed microbial culture cultivated on fermented dairy manure.

Hanson Andrea J AJ Guho Nicholas M NM Paszczynski Andrzej J AJ Coats Erik R ER

Applied microbiology and biotechnology 20160505 18

Polyhydroxyalkanoates (PHAs) are bio-based, biodegradable polyesters that can be produced from organic-rich waste streams using mixed microbial cultures (MMCs). To maximize PHA production, MMCs are enriched for bacteria with a high polymer storage capacity through the application of aerobic dynamic feeding (ADF) in a sequencing batch reactor (SBR), which consequently induces a feast-famine metabolic response. Though the feast-famine response is generally understood empirically at a macro-level, ...[more]

PMID: 27147532

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