Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Brain
SUBMITTER: Andreas Roempp
LAB HEAD: Andreas Römpp
PROVIDER: PXD003172 | Pride | 2018-08-08
REPOSITORIES: Pride
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T019-imagedproteins.pdf | ||||
T019_Recall716.12461.ibd | Other | |||
T019_Recall716.12461.imzML | Other |
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Huber Katharina K Khamehgir-Silz Pegah P Schramm Thorsten T Gorshkov Vladimir V Spengler Bernhard B Römpp Andreas A
Analytical and bioanalytical chemistry 20180801 23
On-tissue digestion has become the preferred method to identify proteins in mass spectrometry (MS) imaging. In this study, we report advances in data acquisition and protein identification for MS imaging after on-tissue digestion. Tryptic peptides in a coronal mouse brain section were measured at 50 μm pixel size and revealed detailed histological structures, e.g., the ependyma (consisting of one to two cell layers), which was confirmed by H&E staining. This demonstrates that MS imaging of trypt ...[more]