Proteomics

Dataset Information

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Proteogenomic analysis in hibernating mammal suggests contribution of skeletal muscle physiology to hibernation phenotype


ABSTRACT: Mammalian hibernation is a strategy employed by many species to survive fluctuations in resource availability and environmental conditions. Hibernating mammals endure conditions of dramatically depressed heart rate, body temperature, and oxygen consumption; yet show no typical pathological response. Because of the high abundance and metabolic cost of skeletal muscle, not only must it adjust to the constraints of hibernation, but is positioned to play a more active role in the initiation and maintenance of the hibernation phenotype. In this study, MS/MS proteomic data were searched against a custom database of transcriptomic and genomic protein predictions built using the platform GalaxyP. This proteogenomic approach allows for a thorough investigation of skeletal muscle protein expression throughout the circannual cycle of the 13-lined ground squirrel. Of the 1,563 proteins identified by these methods, 232 were differentially expressed. Not only do these data support previously reported physiological transitions, they also offer insight into specific mechanisms of how their muscles might be reducing nitrogenous waste, preserving mass and function, and signaling to other tissues. Additionally, the combination of proteomic and transcriptomic data provides unique opportunities for determining the level of post-transcriptional regulation throughout the year and improving the genomic annotation of this non-model organism.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Spermophilus Tridecemlineatus (thirteen-lined Ground Squirrel)

TISSUE(S): Skeletal Muscle

SUBMITTER: Kyle Anderson  

LAB HEAD: Matthew T. Andrews

PROVIDER: PXD003188 | Pride | 2018-07-11

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
PEPTIDE_report_SKM1 Other
Peptide_Summary_with_115_as_denominator_SKM2 Other
Peptide_Summary_with_118_as_denominator_SKM3 Other
Protein_Summary_with_115_SKM1 Other
Protein_Summary_with_115_as_denominator_SKM2 Other
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Publications

Characterizing Cardiac Molecular Mechanisms of Mammalian Hibernation via Quantitative Proteogenomics.

Vermillion Katie L KL   Jagtap Pratik P   Johnson James E JE   Griffin Timothy J TJ   Andrews Matthew T MT  

Journal of proteome research 20151023 11


This study uses advanced proteogenomic approaches in a nonmodel organism to elucidate cardioprotective mechanisms used during mammalian hibernation. Mammalian hibernation is characterized by drastic reductions in body temperature, heart rate, metabolism, and oxygen consumption. These changes pose significant challenges to the physiology of hibernators, especially for the heart, which maintains function throughout the extreme conditions, resembling ischemia and reperfusion. To identify novel card  ...[more]

Publication: 1/2

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