Proteomics

Dataset Information

0

Symbiosis induces widespread changes in the proteome of the model cnidarian Aiptasia


ABSTRACT: Coral reef ecosystems are metabolically founded on the mutualism between corals and photosynthetic dinoflagellates of the genus Symbiodinium. The glass anemone Aiptasia sp. has become a tractable model for this symbiosis. We utilized label-free liquid chromatography electrospray-ionization tandem mass spectrometry to analyze the effects of symbiosis on the proteomes of symbiotic and aposymbiotic Aiptasia. We quantified more than 3,300 proteins in 1,578 protein clusters, with 81 protein clusters showing significantly different expression. Symbiotic anemones showed higher expression of proteins involved in lipid storage and transport, nitrogen transport and cycling, intracellular trafficking, endocytosis and inorganic carbon transport. These changes reflect shifts in host metabolism and energy reserves due to increased organic and inorganic nutritional exchange with the symbionts. Aposymbiotic anemones exhibited increased expression of multiple systems responsible for mediating reactive oxygen stress, suggesting that the host derives direct or indirect protection from oxidative stress while in symbiosis. Aposymbiotic anemones also increased their expression of an array of proteases and chitinases, indicating a metabolic shift from autotrophy to heterotrophy. These results provide a comprehensive Aiptasia proteome with more direct relative quantification of protein abundance than transcriptomic methods, allowing more powerful studies of coral physiology and ecosystem function.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Aiptasia Pulchella (tropical Sea Anemone)

TISSUE(S): Whole Body

SUBMITTER: Clinton Oakley  

LAB HEAD: Simon Davy

PROVIDER: PXD003202 | Pride | 2017-02-22

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
AvS_A1.msf Msf
AvS_A1.mzid Mzid
AvS_A1.mzid_AvS_A1.MGF Mzid
AvS_A1.raw Raw
AvS_A1_150304041916.msf Msf
Items per page:
1 - 5 of 101
altmetric image

Publications

Symbiosis induces widespread changes in the proteome of the model cnidarian Aiptasia.

Oakley Clinton A CA   Ameismeier Michael F MF   Peng Lifeng L   Weis Virginia M VM   Grossman Arthur R AR   Davy Simon K SK  

Cellular microbiology 20160205 7


Coral reef ecosystems are metabolically founded on the mutualism between corals and photosynthetic dinoflagellates of the genus Symbiodinium. The glass anemone Aiptasia sp. has become a tractable model for this symbiosis, and recent advances in genetic information have enabled the use of mass spectrometry-based proteomics in this model. We utilized label-free liquid chromatography electrospray-ionization tandem mass spectrometry to analyze the effects of symbiosis on the proteomes of symbiotic a  ...[more]

Similar Datasets

2019-05-27 | PXD009253 | Pride
2018-03-14 | PXD004257 | Pride
2019-11-06 | PXD014076 | Pride
2024-01-18 | PXD045585 | Pride
2024-01-18 | PXD045587 | Pride
2016-12-22 | GSE92695 | GEO
2011-06-01 | GSE22360 | GEO
2011-06-01 | E-GEOD-22375 | biostudies-arrayexpress
2011-06-01 | GSE22361 | GEO
2011-06-01 | E-GEOD-22360 | biostudies-arrayexpress