Proteomics

Dataset Information

0

Whole saliva baseline proteome profile - head and neck cancer


ABSTRACT: Oral mucositis (OM) is a common, painful and often treatment-limiting side effect of radiotherapy (RT) for head and neck cancer (HNC) patients. Unstimulated saliva was collected before the first radiotherapy application in 50 HNC patients. 41 out of 50 patients developed OM (grade III) during radiotherapy, of which 14 patients even displayed an early OM (grade III) at low radiation dose of 30 Gy. Nine patients did not develop OM (grade III). Using an LC-MS/MS approach 5,323 tryptic peptides were assigned to 487 distinct proteins (≥2 peptides) in the data set. The levels of 48 proteins differed significantly (p<0.05) between patients developing OM or not. 17 proteins displayed increased levels (≥1.3-fold) and 31 proteins decreased in level in OM, respectively. Furthermore, using partial least square analysis proteins patterns could be used to distinguish subjects which did not develop grade III OM even after 70 Gy total dose (n=9) and those displaying early OM (grade III at <30 Gy total dose, n=14). Using leave one out cross validation 37 of 41 patients (90%) developing OM could be correctly assigned indicating that prognostic proteome signatures may help to identify patients that should be specifically monitored to increase overall effectiveness of RT treatment.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

SUBMITTER: Nico Jehmlich  

LAB HEAD: Uwe Völker

PROVIDER: PXD003230 | Pride | 2016-07-04

REPOSITORIES: Pride

altmetric image

Publications

Differences in the whole saliva baseline proteome profile associated with development of oral mucositis in head and neck cancer patients undergoing radiotherapy.

Jehmlich Nico N   Stegmaier Petra P   Golatowski Claas C   Salazar Manuela Gesell MG   Rischke Christian C   Henke Michael M   Völker Uwe U  

Journal of proteomics 20150519


Oral mucositis (OM) is a common, painful and often treatment-limiting side effect of radiotherapy (RT) for head and neck cancer (HNC) patients. Unstimulated saliva was collected before the first radiotherapy application in 50 HNC patients. 41 out of 50 patients developed OM (grade III) during radiotherapy, of which 14 patients even displayed an early OM (grade III) at a low radiation dose of 30Gy. Nine patients did not develop OM (grade III). Using an LC-MS/MS approach 5323 tryptic peptides were  ...[more]

Publication: 1/2

Similar Datasets

2023-07-10 | PXD033603 | Pride
2023-07-10 | PXD033591 | Pride
2012-04-24 | E-GEOD-29298 | biostudies-arrayexpress
2023-05-10 | PXD035565 | Pride
2011-09-08 | E-GEOD-24411 | biostudies-arrayexpress
2011-09-08 | E-GEOD-24037 | biostudies-arrayexpress
2015-01-15 | E-GEOD-64951 | biostudies-arrayexpress
2022-05-19 | PXD030108 | Pride
2011-05-12 | E-GEOD-29220 | biostudies-arrayexpress
2011-09-08 | E-GEOD-24064 | biostudies-arrayexpress