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BRAF interactome - Phospho-proteomic analyses of B-Raf protein complexes reveal new regulatory principles

ABSTRACT: SILAC-based analysis of BRAF interaction partners in DT40 chicken cells and mouse embryonic fibroblasts (MEF)

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Gallus Gallus (chicken) Mus Musculus (mouse)

TISSUE(S): B Cell, Fibroblast

DISEASE(S): Colon Cancer

SUBMITTER: Joern Dengjel

LAB HEAD: Joern Dengjel

PROVIDER: PXD003256 | Pride | 2016-07-13

REPOSITORIES: Pride

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			Action	DRS
	20100112_T_AM1.RAW	Raw
	20100112_T_AM2.RAW	Raw
	20100112_T_AM3.RAW	Raw
	20100112_T_AM4.RAW	Raw
	20100112_T_AM5.RAW	Raw

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Phospho-proteomic analyses of B-Raf protein complexes reveal new regulatory principles.

Eisenhardt Anja E AE Sprenger Adrian A Röring Michael M Herr Ricarda R Weinberg Florian F Köhler Martin M Braun Sandra S Orth Joachim J Diedrich Britta B Lanner Ulrike U Tscherwinski Natalja N Schuster Simon S Dumaz Nicolas N Schmidt Enrico E Baumeister Ralf R Schlosser Andreas A Dengjel Jörn J Brummer Tilman T

Oncotarget 20160501 18

B-Raf represents a critical physiological regulator of the Ras/RAF/MEK/ERK-pathway and a pharmacological target of growing clinical relevance, in particular in oncology. To understand how B-Raf itself is regulated, we combined mass spectrometry with genetic approaches to map its interactome in MCF-10A cells as well as in B-Raf deficient murine embryonic fibroblasts (MEFs) and B-Raf/Raf-1 double deficient DT40 lymphoma cells complemented with wildtype or mutant B-Raf expression vectors. Using a m ...[more]

PMID: 27034005

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