Proteomics

Dataset Information

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Chicken plasma proteome LPS - Proteomic Changes in Chicken Plasma Induced by Salmonella typhimurium Lipopolysaccharides


ABSTRACT: Lipopolysaccharides (LPS) are cell wall components of gram-negative bacteria and endotoxins that cause inflammation and sickness in vertebrate animals. Our objective to identify the plasma proteomic changes in an avian model of inflammation. Chickens were treated with either saline or LPS and the blood was collected 24 hours after injection. The pooled plasma samples were depleted of high abundant proteins, analyzed by Matrix assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) and liquid chromatography–tandem mass spectrometry (LC-MS/MS). MALDI-TOF analyses showed an increase in fibrinogen beta derived peptide and decrease in apolipoprotein AII- isoform X1 derived peptide in LPS samples. Label free quantitation of LC-MS/MS spectra revealed an increase in alpha-1 acid glycoprotein, chemokine-CCLI10, and cathelicidin-2 and decrease in interferon stimulated gene-12-2 in the LPS group. These differentially expressed proteins are associated with inflammation, cytokines, immunomodulation, and defense which are useful as candidate biomarkers of infection and inflammation.

INSTRUMENT(S): Bruker Daltonics amaZon series

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Blood Cell, Blood Plasma

DISEASE(S): Systemic Inflammatory Response Syndrome

SUBMITTER: Balamurugan Packialakshmi  

LAB HEAD: Narayan C Rath

PROVIDER: PXD003335 | Pride | 2016-08-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
CTRL1.mzXML Mzxml
CTRL1.xtan.xml-pride.pride.mgf.gz Mgf
CTRL1.xtan.xml-pride.pride.mztab.gz Mztab
CTRL1.xtan.xml-pride.xml.gz Xml
CTRL2.mzXML Mzxml
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Publications

Proteomic Changes in Chicken Plasma Induced by Salmonella typhimurium Lipopolysaccharides.

Packialakshmi Balamurugan B   Liyanage Rohana R   Lay Jackson O JO   Makkar Sarbjeet K SK   Rath Narayan C NC  

Proteomics insights 20160331


Lipopolysaccharides (LPS) are cell wall components of Gram-negative bacteria that produce inflammation and sickness in higher animals. The objective was to identify plasma proteomic changes in an avian model of inflammation. Chickens were treated with either saline or LPS, and blood was collected at 24 hours postinjection. The pooled plasma samples were depleted of high-abundant proteins and analyzed by matrix-assisted laser desorption ionization (MALDI)-time-of-flight mass spectrometry and liqu  ...[more]

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