Dataset Information

Xanthine Oxidoreductase mediates milk fat globule secretion.

ABSTRACT: Analysis of the milk fat globule proteome from mice with a mammary-specific knockout of Xanthine Oxidoreductase.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Matthew Wither

LAB HEAD: Jenifer Monks

PROVIDER: PXD003336 | Pride | 2017-05-31

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20140422_Jmonks_MFG_MGKO_163-_run1_ITMSms2cid.mgf	Mgf
	20140422_Jmonks_MFG_MGKO_163-_run1_ITMSms2cid.pride.mgf.gz	Mgf
	20140422_Jmonks_MFG_MGKO_163-_run2_ITMSms2cid.mgf	Mgf
	20140422_Jmonks_MFG_MGKO_163-_run2_ITMSms2cid.pride.mgf.gz	Mgf
	20140422_Jmonks_MFG_MGKO_163R_run1_ITMSms2cid.mgf	Mgf

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Publications

Xanthine oxidoreductase mediates membrane docking of milk-fat droplets but is not essential for apocrine lipid secretion.

Monks Jenifer J Dzieciatkowska Monika M Bales Elise S ES Orlicky David J DJ Wright Richard M RM McManaman James L JL

The Journal of physiology 20160803 20

<h4>Key points</h4>Xanthine oxidoreductase (XOR) modulates milk lipid secretion and lactation initiation. XOR is required for butyrophilin1a1 clustering in the membrane during milk lipid secretion. XOR mediates apical membrane reorganization during milk lipid secretion. Loss of XOR delays milk fat globule secretion. XOR loss alters the proteome of milk fat globules.<h4>Abstract</h4>Apocrine secretion is utilized by epithelial cells of exocrine glands. These cells bud off membrane-bound particles ...[more]

PMID: 27357166

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Project description:Objective: Procyanidins are polyphenolic bioactive compounds that exert beneficial effects against obesity and its related diseases. The aim of this study was to evaluate whether the supplementation with low doses of a grape seed procyanidin extract (GSPE) to dams during pre and postnatal periods has biological effects on their offspring at youth. Design: The metabolic imprinting effect of GSPE was evaluated in 30 days-old male offspring of four groups of rats that were fed either a standard diet (STD) or a high-fat diet (HFD) and supplemented with either GSPE at 25 mg per kg of body weight/day or vehicle during pregnancy and lactation. Results: A significant increase in the adiposity index and in the weight of all the white adipose tissue depots studied (retroperitoneal â??RWAT-, mesenteric â??MWAT-, epididymal â??EWAT- and inguinal â??IWAT-) was observed in offspring of dams fed with a HFD and treated with GSPE (HFT group), compared to the offspring of dams fed with the same diet and that do not received procyanidins (HF group). HFT animals also showed a higher number of cells in the EWAT, a sharply decrease of the circulating levels of monocyte chemoattractant protein-1 (MCP-1) as well as a moderate, but significant, decrease of plasma glycerol levels. The transcriptomic analysis performed in the EWAT showed 238 genes differentially expressed between HF and HFT animals, covering an entire range of processes related with the immune function and the inflammatory response (the metabolic pathway mainly reflected in the EWAT), adipose tissue remodeling and function, lipid and glucose homeostasis and metabolism of methyl groups. Conclusion: GSPE treatment to dams fed a HFD during pregnancy and lactation increases adiposity, decreases the circulating levels of MCP-1 and modulates the expression of key genes involved in the adipose tissue metabolism of their offspring. The microarray study was performed with the EWAT RNA samples of rats from the HF and the HFT groups (n=8 animals each).

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