Dataset Information

Tandem affinity purification and Mass spectrometry (TAP-MS) analysis of PCH1’s associated proteins in various Arabidopsis genetic backgrounds.

ABSTRACT: Here we describe a series TAP-MS analyses in various mutant backgrounds of Arabidopsis thaliana to co-purify any PCH1’s associated proteins, using tagged PCH1 as bait (PCH1-His6-Flag3, either overexpressed or under the control of its own promoter). By combining TAP-MS with genetics, we investigate the in-vivo dependence of several proteins for maintaining the association of PCH1 with its interacting proteins. Our results refine the protein-protein interaction network of an existing interactome containing phytochromes, the COP1-SPAs complex and the ELF3-ELF4-LUX complex.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Sophe Alvarez

LAB HEAD: Meter Nusinow

PROVIDER: PXD003352 | Pride | 2016-02-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20131008_MN_elf3_2 (F016253).mzid_20131008_MN_elf3_2_(F016253).pride.mgf.gz	Mzid
	20131008_MN_elf3_2 (F016253).pride.mztab.gz	Mztab
	20131008_MN_elf3_2.raw	Raw
	20131008_MN_elf3_2F016253.mzid.gz	Mzid
	20131008_MN_elf3_2F016253.mzid_20131008_MN_elf3_2_F016253.MGF	Mzid

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Publications

PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth in Arabidopsis.

Huang He H Yoo Chan Yul CY Bindbeutel Rebecca R Goldsworthy Jessica J Tielking Allison A Alvarez Sophie S Naldrett Michael J MJ Evans Bradley S BS Chen Meng M Nusinow Dmitri A DA

eLife 20160203

Plants react to seasonal change in day length through altering physiology and development. Factors that function to harmonize growth with photoperiod are poorly understood. Here we characterize a new protein that associates with both circadian clock and photoreceptor components, named PHOTOPERIODIC CONTROL OF HYPOCOTYL1 (PCH1). pch1 seedlings have overly elongated hypocotyls specifically under short days while constitutive expression of PCH1 shortens hypocotyls independent of day length. PCH1 pe ...[more]

PMID: 26839287

Dataset Information

Tandem affinity purification and Mass spectrometry (TAP-MS) analysis of PCH1’s associated proteins in various Arabidopsis genetic backgrounds.

Dataset's files

Publications

PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth in Arabidopsis.

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