Dataset Information

Homogenization of tissues via picosecond-infrared laser (PIRL) ablation: Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization

ABSTRACT: Tissue samples from human tonsils and rat pancreas were subjected to a novel laser-based homogenization method, the PIRL-DIVE homogenization, and to a conventional mechanical tissue homogenization procedure (bead mill and a cryo-grinder). The protein species composition of the protein extracts were compared by differential proteome analysis using two-dimensional gel electrophoresis followed by LC-MS/MS analysis and one-dimensional gel electrophoresis (SDS-PAGE) followed by quantitative LC-MS/MS analysis.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Rattus Norvegicus (rat) Homo Sapiens (human)

TISSUE(S): Tonsil, Pancreas

SUBMITTER: Marcus Wurlitzer

LAB HEAD: Hartmut Schlüter

PROVIDER: PXD003434 | Pride | 2016-01-25

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	1DE_Pancreas-1-3_MaxQuant.7z	Other
	1DE_Pancreas-1_DH_01.mgf.7z	Mgf
	1DE_Pancreas-1_DH_01.raw	Raw
	1DE_Pancreas-1_DH_02.mgf.7z	Mgf
	1DE_Pancreas-1_DH_02.raw	Raw

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Homogenization of tissues via picosecond-infrared laser (PIRL) ablation: Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization.

Kwiatkowski M M Wurlitzer M M Krutilin A A Kiani P P Nimer R R Omidi M M Mannaa A A Bussmann T T Bartkowiak K K Kruber S S Uschold S S Steffen P P Lübberstedt J J Küpker N N Petersen H H Knecht R R Hansen N O NO Zarrine-Afsar A A Robertson W D WD Miller R J D RJD Schlüter H H

Journal of proteomics 20160108

Posttranslational modifications and proteolytic processing regulate almost all physiological processes. Dysregulation can potentially result in pathologic protein species causing diseases. Thus, tissue species proteomes of diseased individuals provide diagnostic information. Since the composition of tissue proteomes can rapidly change during tissue homogenization by the action of enzymes released from their compartments, disease specific protein species patterns can vanish. Recently, we describe ...[more]

PMID: 26778141

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