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Nuclear nucleolin interactome analysis

ABSTRACT: Nucleolin (NCL) is a major component of the cell nucleolus, which has the ability to rapidly shuttle to several other cell’s compartments. NCL has been shown to play important roles in a variety of essential functions among which ribosome biogenesis, gene expression and cell growth. However, the precise mechanisms underlying NCL functions are still unclear. Our study aimed to provide new information on NCL functions via the identification of its nuclear interacting partners. Using an interactomics approach, we identified 140 binding partners of NCL, among which, a 100 of them, were specifically found associated to NCL independently of the presence of RNA.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yohann Couté

LAB HEAD: Christophe Bruley

PROVIDER: PXD003554 | Pride | 2016-04-11

REPOSITORIES: Pride

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			Action	DRS
	VELOS1718.raw	Raw
	VELOS1722.raw	Raw
	VELOS18807.raw	Raw
	VELOS18809.raw	Raw
	VELOS18811.raw	Raw

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Publications

Nuclear Functions of Nucleolin through Global Proteomics and Interactomic Approaches.

Salvetti Anna A Couté Yohann Y Epstein Alberto A Arata Loredana L Kraut Alexandra A Navratil Vincent V Bouvet Philippe P Greco Anna A

Journal of proteome research 20160421 5

Nucleolin (NCL) is a major component of the cell nucleolus, which has the ability to rapidly shuttle to several other cells' compartments. NCL plays important roles in a variety of essential functions, among which are ribosome biogenesis, gene expression, and cell growth. However, the precise mechanisms underlying NCL functions are still unclear. Our study aimed to provide new information on NCL functions via the identification of its nuclear interacting partners. Using an interactomics approach ...[more]

PMID: 27049334

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Characterizations of HSP90-interacting complex in renal cells using tandem affinity purification and its potential role in kidney stone formation

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