Tissue-specific Proteogenomic Analysis of Plutella xylostella Larval Midgut Using a Multi-algorithme Pipeline, Part 1/5

ABSTRACT: Plutella xylostella is the major cosmopolitan pest of brassica and other crucifer crops,the larval midgut of which is a dynamic organ that interfaces with a diversearray of physiological and toxicological processes.The draft sequence of the P.xylostella genome was recently released,but its annotation remains chanllenging because of the low coverage of this branch of life.Peptide sequencing by computational assignment of tandem mass spectra to a database of putative protein sequences provides an independent approach to confirm or refute protein prediction,which has been termed proteogenomics.In this study,we carried out an in-depth proteogenomic analysis using shotgun HPLC-ESI-MS/MS approach with a multi-algorithme peipline to complement genome annotation in the P.xylostella larval midgut.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Plutella Xylostella (diamondback Moth) (plutella Maculipennis)

TISSUE(S): Midgut

SUBMITTER: Xun Zhu  

LAB HEAD: Department of Entomology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

PROVIDER: PXD003564 | Pride | 2016-04-21

REPOSITORIES: Pride