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Pasteurella multocida Hfq mutant

ABSTRACT: Quantitative proteomics comparison wt and Hfq mutant Pasteurella multocida strains.

OTHER RELATED OMICS DATASETS IN: PRJNA311305

INSTRUMENT(S): Q Exactive

ORGANISM(S): Pasteurella Multocida Subsp. Multocida Str. Pm70

SUBMITTER: Oded Kleifeld

LAB HEAD: John Boyce

PROVIDER: PXD003586 | Pride | 2016-03-14

REPOSITORIES: Pride

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			Action	DRS
	MaxQuantOutput.tar.gz	Other
	OK20140220_AW_wtHmutL.raw	Raw
	OK20140220_AW_wtLmutH.raw	Raw
	OK20140503_AW_mutHwtL_CA.raw	Raw
	OK20140503_AW_mutHwtL_DB.raw	Raw

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Publications

The RNA-Binding Chaperone Hfq Is an Important Global Regulator of Gene Expression in Pasteurella multocida and Plays a Crucial Role in Production of a Number of Virulence Factors, Including Hyaluronic Acid Capsule.

Mégroz Marianne M Kleifeld Oded O Wright Amy A Powell David D Harrison Paul P Adler Ben B Harper Marina M Boyce John D JD

Infection and immunity 20160422 5

The Gram-negative bacterium Pasteurella multocida is the causative agent of a number of economically important animal diseases, including avian fowl cholera. Numerous P. multocida virulence factors have been identified, including capsule, lipopolysaccharide (LPS), and filamentous hemagglutinin, but little is known about how the expression of these virulence factors is regulated. Hfq is an RNA-binding protein that facilitates riboregulation via interaction with small noncoding RNA (sRNA) molecule ...[more]

PMID: 26883595

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Project description:The small RNAs associated with protein Hfq constitute one of the largest classes of post-transcriptional regulators known to date. Most previously investigated members of this class are encoded by conserved free-standing genes. Here, deep sequencing of Hfq-bound transcripts from multiple stages of growth of Salmonella Typhimurium revealed a plethora of new small RNA species from within mRNA loci, including DapZ which overlaps with the 3’ region of the biosynthetic gene, dapB. Synthesis of the DapZ small RNA is independent of DapB protein synthesis, and controlled by HilD, the master regulator of Salmonella invasion genes. DapZ carries a short G/U-rich domain similar to that of the globally acting GcvB small RNA, and uses GcvB-like seed pairing to repress translation of the major ABC transporters, DppA and OppA. This exemplifies double functional output from an mRNA locus by the production of both a protein and an Hfq-dependent trans-acting RNA. Our atlas of Hfq targets suggests that the 3’ regions of mRNA genes constitute a rich reservoir to feed the Hfq network with new regulatory small RNAs. Hfq-associated RNAs were systemically analyzed in Salmonella at 7 different growth stages in standard labortory condition (LB)

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Comparison of the transcriptomes of a Pasteurella multocida VP161 wild-type strain with a Pasteurella multocida VP161 hfq mutant

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