Proteomics

Dataset Information

0

Shotgun proteomics of a rice elongating internode at boosting stage


ABSTRACT: Stem internodes of grasses function in mechanical support, transport, and, in some species are a major sink organ for carbon in the form of cell wall polymers. To establish the rice elongating internode as a model for secondary cell wall development, we conducted cell wall composition, proteomic and metabolomic analyses of the second rice internode at booting stage. We measured major secondary cell wall components along eight segments of an elongating internode. Cellulose, lignin, and xylose increase as a percentage of cell wall material from the younger to the older internode segments, indicating active cell wall synthesis. With the whole elongating internode, we measured peptides via liquid-chromatography mass spectrometry (LC-MS) following trypsin digestion of size fractionated proteins. This identified a total of 3249 protein groups with at least two unique peptides, including many glycosyltransferases, acyltransferases, glycohyrolases, cell wall-localized proteins, and protein kinases that have or may have functions in cell wall biosynthesis or remodeling. In addition, GO over-representation analysis and KO pathway analysis indicate many proteins involved in biosynthetic processes, especially the synthesis of secondary metabolites such as phenylpropanoids, flavonoids, and tepenoids. Therefore, we also used LC-MS to measure methanol-extracted secondary metabolites from the whole internode at the elongation stage and three post-elongation stages, and from leaf and root at the second post-elongation stage. The results indicate secondary metabolites in stems are distinct from those of roots and leaves, and show different profiles during stem development. This study fills a void of knowledge of proteomics and metabolomics data for grass stems, especially of rice, and provides baseline knowledge for more detailed studies of cell wall synthesis and other biological processes during internode development. This and future work is aimed at optimizing stem development and cell wall composition of grasses to improve agronomic properties and biofuel production.

INSTRUMENT(S): LTQ Orbitrap, Synapt MS

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Plant Cell, Shoot

SUBMITTER: Fan Lin  

LAB HEAD: Laura E. Bartley

PROVIDER: PXD003676 | Pride | 2017-08-04

REPOSITORIES: Pride

altmetric image

Publications

Proteomics Coupled with Metabolite and Cell Wall Profiling Reveal Metabolic Processes of a Developing Rice Stem Internode.

Lin Fan F   Williams Brad J BJ   Thangella Padmavathi A V PAV   Ladak Adam A   Schepmoes Athena A AA   Olivos Hernando J HJ   Zhao Kangmei K   Callister Stephen J SJ   Bartley Laura E LE  

Frontiers in plant science 20170713


Internodes of grass stems function in mechanical support, transport, and, in some species, are a major sink organ for carbon in the form of cell wall polymers. This study reports cell wall composition, proteomic, and metabolite analyses of the rice elongating internode. Cellulose, lignin, and xylose increase as a percentage of cell wall material along eight segments of the second rice internode (internode II) at booting stage, from the younger to the older internode segments, indicating active c  ...[more]

Similar Datasets

2011-03-06 | E-GEOD-24014 | biostudies-arrayexpress
2011-03-06 | GSE24014 | GEO
| PRJNA766080 | ENA
2009-06-08 | E-GEOD-13602 | biostudies-arrayexpress
2006-11-19 | GSE5021 | GEO
2020-11-20 | PXD020160 | Pride
2010-04-01 | E-MEXP-2311 | biostudies-arrayexpress
2016-02-22 | E-GEOD-77153 | biostudies-arrayexpress
2020-11-29 | GSE153705 | GEO
2012-01-26 | E-MEXP-3525 | biostudies-arrayexpress