Proteomics

Dataset Information

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Bubalus bubalis seminal plasma


ABSTRACT: The domestic buffalo (Bubalus bubalis) has presented an important role in the livestock industry, contributing to milk and meat production worldwide, especially in developing countries. However, little is known about its reproductive particularities. Studies regarding protein composition of buffalo SP are still limited and a complete mapping of buffalo SP proteins is still lacking in the literature. Hence, a comprehensive study of SP proteome is of great importance to better understand the mechanisms involved in male reproduction and to optimize the reproductive biotechnologies of farm animal species. Therefore, the aim of this study is to describe for the first time the Bubalus bubalis seminal plasma proteome using a label free shotgun HDMS approach. This type of analysis is interesting since it yields a high number of detected proteins, generating a dataset that is useful for further characterizing the buffalo SP.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Bubalus Bubalis

TISSUE(S): Seminal Plasma

SUBMITTER: Henrique César Pereira Figueiredo  

LAB HEAD: Henrique César Pereira Figueiredo

PROVIDER: PXD003728 | Pride | 2018-06-25

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20151020_41_F001.raw.zip Raw
20151020_41_F001searchresult.zip Other
20151020_41_F002.raw.zip Raw
20151020_41_F002searchresult.zip Other
20151020_41_F003.raw.zip Raw
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Publications


The study aimed to describe the Bubalus bubalis seminal plasma proteome using a label-free shotgun UDMS<sup>E</sup> approach. A total of 859 nonredundant proteins were identified across five biological replicates with stringent identification. Proteins specifically related to sperm maturation and protection, capacitation, fertilization and metabolic activity were detected in the buffalo seminal fluid. In conclusion, we provide a comprehensive proteomic profile of buffalo seminal plasma, which es  ...[more]

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