Dataset Information

Parallel reaction monitoring using quadrupole-orbitrap mass spectrometer: principle and applications

ABSTRACT: The PRM dataset corresponds to the raw data files supporting the comparison between conventional database search using Mascot and spectral matching strategy (Figure 3). The PRM and DDA raw data files support the comparison between these two acquisition methods using the spectral matching strategy to corroborate the identity of the peptides (Figure 4D). The data were generated from the triplicate analyses of a dilution series of 778 SIL peptides spiked in a yeast tryptic digest at concentrations ranging from 50 amol/µL to 37 fmol/µL.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Adèle Bourmaud

LAB HEAD: Bruno Domon

PROVIDER: PXD003781 | Pride | 2016-05-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	DDA_Yeast_300ng_778SIL_12000amol_1.raw	Raw
	DDA_Yeast_300ng_778SIL_12000amol_2.raw	Raw
	DDA_Yeast_300ng_778SIL_12000amol_3.raw	Raw
	DDA_Yeast_300ng_778SIL_1400amol_1.raw	Raw
	DDA_Yeast_300ng_778SIL_1400amol_2.raw	Raw

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Parallel reaction monitoring using quadrupole-Orbitrap mass spectrometer: Principle and applications.

Bourmaud Adele A Gallien Sebastien S Domon Bruno B

Proteomics 20160527 15-16

Targeted mass spectrometry-based approaches are nowadays widely used for quantitative proteomics studies and more recently have been implemented on high resolution/accurate mass (HRAM) instruments resulting in a considerable performance improvement. More specifically, the parallel reaction monitoring technique (PRM) performed on quadrupole-Orbitrap mass spectrometers, leveraging the high resolution and trapping capabilities of the instrument, offers a clear advantage over the conventional select ...[more]

PMID: 27145088

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