Proteomics

Dataset Information

0

Mouse stem cell SWATH-MS

ABSTRACT: Adult stem cells (SCs) are essential for tissue maintenance and regeneration yet are susceptible to SC senescence during aging. Here we demonstrate the importance of cellular NAD+ level and its impact on mitochondrial activity as a pivotal switch to modulate muscle stem cell (MuSC) senescence. Importantly, the induction of the mitochondrial unfolded protein response (UPRmt) and of prohibitin proteins, subsequent to increasing cellular NAD+ with the precursor nicotinamide riboside (NR), rejuvenates MuSCs in aged mice. NR also prevents MuSCs senescence in Mdx mice, a mouse model of muscular dystrophy. Extending these observations to other SC pools and on the organism as a whole, we demonstrate that NR delays neural stem cell (NSC) and melanocyte stem cell (McSC) senescence, while also increasing mouse lifespan. Strategies that conserve cellular NAD+ could therefore be utilized to reprogram dysfunctional SCs in aging and disease to improve lifespan in mammals

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Stem Cell, Muscle

SUBMITTER: Yibo Wu  

LAB HEAD: Ruedi Aebersold

PROVIDER: PXD003865 | Pride | 2022-02-22

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
InterProphet.pep.xml Pepxml
ywu_L151128_001_SW.zip Other
ywu_L151128_002_SW.zip Other
ywu_L151128_003_SW.zip Other
ywu_L151128_004_SW.zip Other
Items per page:
1 - 5 of 5
altmetric image

Publications

NAD⁺ repletion improves mitochondrial and stem cell function and enhances life span in mice.

Zhang Hongbo H   Ryu Dongryeol D   Wu Yibo Y   Gariani Karim K   Wang Xu X   Luan Peiling P   D'Amico Davide D   Ropelle Eduardo R ER   Lutolf Matthias P MP   Aebersold Ruedi R   Schoonjans Kristina K   Menzies Keir J KJ   Auwerx Johan J  

Science (New York, N.Y.) 20160428 6292

Adult stem cells (SCs) are essential for tissue maintenance and regeneration yet are susceptible to senescence during aging. We demonstrate the importance of the amount of the oxidized form of cellular nicotinamide adenine dinucleotide (NAD(+)) and its effect on mitochondrial activity as a pivotal switch to modulate muscle SC (MuSC) senescence. Treatment with the NAD(+) precursor nicotinamide riboside (NR) induced the mitochondrial unfolded protein response and synthesis of prohibitin proteins,  ...[more]

Similar Datasets

Transcriptomics Nicotinamide riboside augments the human skeletal muscle NAD+ 1 metabolome and induces transcriptomic and anti-inflammatory signatures in aged subjects: a placebo-controlled, randomized trial
2019-08-29 | GSE133261 | GEO
Transcriptomics NAD+ supplementation counteracts senescence mediated by STING in Ataxia Telangiectasia models by improving mitophagy (Human)
2021-05-25 | GSE152287 | GEO
Transcriptomics NAD+ supplementation counteracts senescence mediated by STING in Ataxia Telangiectasia models by improving mitophagy (Mouse)
2021-05-25 | GSE152286 | GEO
Proteomics Niche stiffening compromises stem cell potential during aging by reducing chromatin accessibility at bivalent promoters
2021-05-07 | PXD018352 | Pride
Transcriptomics The transcription Factor Slug represses p16Ink4a and regulates murine muscle stem cell aging
2019-03-20 | GSE128507 | GEO
Proteomics Proteomic analysis of small extracellular vesicles derived from human ESCs
2021-08-30 | PXD018655 | Pride
Proteomics Restoration of CPEB4 Prevents Muscle Stem Cell Senescence during Aging
2023-06-26 | PXD041614 | Pride
Proteomics Mouse Muscle Stem Cell Proteome during Activation and CPEB1 Dependent Proteome in Mouse Muscle Stem Cell
2021-11-05 | PXD018865 | Pride
Genomics NAD+ controls circadian reprogramming through PER2 nuclear translocation to counter aging
2020-05-04 | GSE133989 | GEO
Genomics Histone modifications in activated and freshly isolated mouse muscle stem cells by ChIP-seq
2022-08-12 | GSE189046 | GEO