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Venomics of the East African green mamba and black mamba, highlights the complexity of their toxin arsenal

ABSTRACT: Complementary bottom-up MS/MS analyses for the venom phenotype map for the eastern green mamba and black mamba

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Dendroaspis Angusticeps (eastern Green Mamba) (naja Angusticeps) Dendroaspis Polylepis Polylepis (black Mamba)

TISSUE(S): Venom

SUBMITTER: Daniel Petras

LAB HEAD: Juan J. Calvete

PROVIDER: PXD003876 | Pride | 2016-06-27

REPOSITORIES: Pride

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			Action	DRS
	BM_Peak12.cpsx	Other
	BM_Peak12.mgf	Mgf
	BM_Peak12.mzid.gz	Mzid
	BM_Peak12.pride.mgf.gz	Mgf
	BM_Peak12.pride.mztab.gz	Mztab

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Top-down venomics of the East African green mamba, Dendroaspis angusticeps, and the black mamba, Dendroaspis polylepis, highlight the complexity of their toxin arsenals.

Petras Daniel D Heiss Paul P Harrison Robert A RA Süssmuth Roderich D RD Calvete Juan J JJ

Journal of proteomics 20160616

We report the characterization, by combination of high-resolution on-line molecular mass and disulfide bond profiling and top-down MS/MS analysis, of the venom proteomes of two congeneric African snake species of medical importance, Dendroaspis angusticeps (green mamba) and D. polylepis (black mamba). Each of these mamba venoms comprised more than two-hundred polypeptides belonging to just a few toxin families. Both venom proteomes are overwhelmingly composed of post-synaptically-acting short- a ...[more]

PMID: 27318176

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