Dataset Information

Shotgun Proteomics of Tomato Fruits: Evaluation and Optimization of Sample Preparation Methods and Mass Spectrometric Parameters

ABSTRACT: Optimization of sample preparation protocols and mass spectrometric parameters is essential for adequate proteome coverage. Though several protocols are available for 2D electrophoresis-based proteome profiling, very few protocols exist for sample preparation for shotgun proteomics and optimization of mass spectrometric parameters using plant tissues. Here, we report an optimized protocol for shotgun proteomics of tomato fruit, which is a recalcitrant tissue comprising of low concentration of proteins and high percentage of sugars and secondary metabolites

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Plant Cell, Fruit

SUBMITTER: Yellamaraju Sreelakshmi

LAB HEAD: Yellamaraju Sreelakshmi

PROVIDER: PXD003920 | Pride | 2016-06-30

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	AC_RR_1streplicate_fraction1.msf	Msf
	AC_RR_1streplicate_fraction1.raw	Raw
	AC_RR_1streplicate_fraction1.sf3	Other
	AC_RR_1streplicate_fraction2.msf	Msf
	AC_RR_1streplicate_fraction2.raw	Raw

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Shotgun Proteomics of Tomato Fruits: Evaluation, Optimization and Validation of Sample Preparation Methods and Mass Spectrometric Parameters.

Kilambi Himabindu V HV Manda Kalyani K Sanivarapu Hemalatha H Maurya Vineet K VK Sharma Rameshwar R Sreelakshmi Yellamaraju Y

Frontiers in plant science 20160629

An optimized protocol was developed for shotgun proteomics of tomato fruit, which is a recalcitrant tissue due to a high percentage of sugars and secondary metabolites. A number of protein extraction and fractionation techniques were examined for optimal protein extraction from tomato fruits followed by peptide separation on nanoLCMS. Of all evaluated extraction agents, buffer saturated phenol was the most efficient. In-gel digestion [SDS-PAGE followed by separation on LCMS (GeLCMS)] of phenol-e ...[more]

PMID: 27446192

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