Dataset Information

Identification of A. thaliana 2-Cys peroxiredoxins interacting proteins

ABSTRACT: Peroxiredoxins are ubiquitous thiol-dependent peroxidases for which chaperone and signaling roles have been reported in various types of organisms in the last years. In plants, the peroxidase function of the two typical plastidial 2-Cys peroxiredoxins (2-Cys PRX A and B) has been highlighted while the other functions, particularly in ROS-dependent signal transduction pathways, are still elusive due notably to the lack of knowledge on interacting partners. Using an ex vivo approach based on co-immunoprecipitation of leaf extracts from Arabidopsis thaliana wild-type and mutant plants lacking 2-Cys PRX expression followed by mass spectrometry-based proteomics, 158 proteins were found associated to 2-Cys PRXs, notably already known partners like thioredoxin-related electron donors (Chloroplastic Drought-induced Stress Protein of 32 kDa, Atypical Cysteine Histidine rich Thioredoxin 2) and enzymes involved in chlorophyll synthesis (Protochlorophyllide OxidoReductase B) or carbon metabolisms (Fructose-1,6-BisPhosphatase), validating the relevance of this approach.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Yohann Couté

LAB HEAD: Christophe Bruley

PROVIDER: PXD003923 | Pride | 2016-10-11

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	2-Cys PRXs interactome analysis.pride.mgf.gz	Mgf
	2-Cys PRXs interactome analysis.pride.mztab.gz	Mztab
	2-CysPRXsinteractomeanalysis.xml.gz	Xml
	WT.raw	Raw
	prx.raw	Raw

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Publications

Characterization of the Arabidopsis thaliana 2-Cys peroxiredoxin interactome.

Cerveau Delphine D Kraut Alexandra A Stotz Henrik U HU Mueller Martin J MJ Couté Yohann Y Rey Pascal P

Plant science : an international journal of experimental plant biology 20160710

Peroxiredoxins are ubiquitous thiol-dependent peroxidases for which chaperone and signaling roles have been reported in various types of organisms in recent years. In plants, the peroxidase function of the two typical plastidial 2-Cys peroxiredoxins (2-Cys PRX A and B) has been highlighted while the other functions, particularly in ROS-dependent signaling pathways, are still elusive notably due to the lack of knowledge of interacting partners. Using an ex vivo approach based on co-immunoprecipit ...[more]

PMID: 27717466

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