Dataset Information

Subcellular fractionation in Ramos B-cells

ABSTRACT: In order to increase the proteome coverage, a subfractionation strategy has been performed to obtain proteins belonging to four different cellular compartments (i.e., cytoplasm (CYT), membrane (MB), organelle (ORG), and nucleus (NUC)). Experiments have been done in triplicate.

INSTRUMENT(S): LTQ Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell, Cell Suspension Culture

DISEASE(S): Lymphoma

SUBMITTER: Paula Díez

LAB HEAD: Manuel Fuentes

PROVIDER: PXD003939 | Pride | 2022-02-14

REPOSITORIES: Pride

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Publications

Deepening into Intracellular Signaling Landscape through Integrative Spatial Proteomics and Transcriptomics in a Lymphoma Model.

Landeira-Viñuela Alicia A Díez Paula P Juanes-Velasco Pablo P Lécrevisse Quentin Q Orfao Alberto A De Las Rivas Javier J Fuentes Manuel M

Biomolecules 20211126 12

Human Proteome Project (HPP) presents a systematic characterization of the protein landscape under different conditions using several complementary-omic techniques (LC-MS/MS proteomics, affinity proteomics, transcriptomics, etc.). In the present study, using a B-cell lymphoma cell line as a model, comprehensive integration of RNA-Seq transcriptomics, MS/MS, and antibody-based affinity proteomics (combined with size-exclusion chromatography) (SEC-MAP) were performed to uncover correlations that c ...[more]

PMID: 34944421

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