Dataset Information

Bathymodiolus azoricus host-symbiont proteomics

ABSTRACT: Bathymodiolus azoricus is a deep-sea mussel found in the hydrothermal vent fields of the Mid-Atlantic Ridge. It lives in symbiosis with sulfur- and methane-oxidizing γ-proteobacteria within its gills. In our study, we aimed to understand the metabolic and physiological interconnections between the symbiotic partners. For this purpose, symbionts and host were physically separated using density gradient centrifugation. This procedure yielded a symbiont-enriched gradient pellet fraction and a supernatant fraction enriched in host components. The cytosolic and membrane-associated proteome of both these fractions along with whole gill and foot tissue of the mussel were then investigated through 1D-PAGE LC-MS/MS. Proteins were quantified based on their spectral counts using the NSAF method. For efficient identification, sequences from evolutionarily related endosymbiotic and free-living bacteria and from bivalve host relatives were compiled into a comprehensive protein database. A total of 3178 host and symbiont proteins were identified from all samples.

OTHER RELATED OMICS DATASETS IN: PRJEB8263PRJEB7941PRJEB7943PRJEB8264PRJEB13047

INSTRUMENT(S): LTQ Orbitrap, LTQ Orbitrap Velos

ORGANISM(S): Thiotrophic Endosymbiont Of Bathymodiolus Azoricus (menez Gwen) Methanotrophic Endosymbiont Of Bathymodiolus Azoricus (menez Gwen) Bathymodiolus Azoricus (mussel)

TISSUE(S): Gill, Foot Muscle

SUBMITTER: Ruby Ponnudurai

LAB HEAD: Thomas Schweder, Stephanie Markert

PROVIDER: PXD004061 | Pride | 2016-10-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Bathymodiolus_azoricus_protein_database.faa	Other
	Mudpit_120328_O1_U3_MM_StM_gradient_pellet_01.mzid.gz	Mzid
	Mudpit_120328_O1_U3_MM_StM_gradient_pellet_01.mzid_Mudpit_120328_O1_U3_MM_StM_gradient_pellet_01.MGF	Mzid
	Mudpit_120328_O1_U3_MM_StM_supernatant_01.mzid.gz	Mzid
	Mudpit_120328_O1_U3_MM_StM_supernatant_01.mzid_Mudpit_120328_O1_U3_MM_StM_supernatant_01.MGF	Mzid

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Publications

Abundant toxin-related genes in the genomes of beneficial symbionts from deep-sea hydrothermal vent mussels.

Sayavedra Lizbeth L Kleiner Manuel M Ponnudurai Ruby R Wetzel Silke S Pelletier Eric E Barbe Valerie V Satoh Nori N Shoguchi Eiichi E Fink Dennis D Breusing Corinna C Reusch Thorsten Bh TB Rosenstiel Philip P Schilhabel Markus B MB Becher Dörte D Schweder Thomas T Markert Stephanie S Dubilier Nicole N Petersen Jillian M JM

eLife 20150915

Bathymodiolus mussels live in symbiosis with intracellular sulfur-oxidizing (SOX) bacteria that provide them with nutrition. We sequenced the SOX symbiont genomes from two Bathymodiolus species. Comparison of these symbiont genomes with those of their closest relatives revealed that the symbionts have undergone genome rearrangements, and up to 35% of their genes may have been acquired by horizontal gene transfer. Many of the genes specific to the symbionts were homologs of virulence genes. We di ...[more]

PMID: 26371554

Publication: 1/2

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Project description:Bathymodiolus mussels inhabiting deep-sea hydrothermal vents harbor bacterial symbionts in their gills, which support the animals’ diet. While the basic mechanisms of energy generation and CO2 fixation that drive these symbioses are largely established, details of molecular interactions between the symbiotic partners and adaptations to their respective habitats remain unknown. In this study, we therefore comparatively examined the genomes and proteomes of two Bathymodiolus hosts and their respective symbionts from different geographical locations. Two mussel species were proteomically compared: i) B. thermophilus mussel containing sulfur-oxidizing symbiont from the east pacific rise. thermophilus and ii) B. azoricus containing thiotrophic and methanotrophic symbionts from the mid-atlantic ridge. Symbionts (for both species) and host components (for B. azoricus) were selectively enriched using a multi-step centrifugation procedure. Enriched host and symbiont fractions along with unenriched gill foot tissue were subject to in-depth semi-quantitative proteomic analyses using the orbitrap and velos mass spectrometers. Proteins were quantified based on their spectral counts using the normalized spectral abundance factor (NSAF) method. We identified common strategies of metabolic interactions that provide mutual nutritional support between host and symbionts, such as the detoxification of ambient sulfide by the Bathymodiolus host, which provides a stable thiosulfate reservoir for the thiotrophic symbionts, and a putative amino acid cycling mechanism that could supply the host with symbiont-derived amino acids. A suite of genes and proteins putatively related to virulence or defense functions was particularly abundant in the B. thermophilus symbiont, compared to its symbiont relatives, and may pose a host species-specific adaptation. Our results reveal both, a high degree of integration between the symbiotic partners, and great potential to adapt to the prevailing environment, which facilitate the holobiont’s survival in its hydrothermal vent habitat.