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Pre-40S ribosome biogenesis factor Tsr1 is an inactive structural mimic of translational GTPases

ABSTRACT: Budding yeast Tsr1 is an essential ribosome biogenesis factor that is required for cytoplasmic steps in 40S subunit maturation. S. cerevisiae Tsr1 was expressed as an N-terminal GST fusion protein in E. coli. A mutant Tsr1ΔNΔloop was generated in which residues 410 to 476 of Tsr1 were replaced with a short glycine and serine rich sequence; in addition, the N-ternimal 45 amino acid residues were replaced by five amino acid residues “GPDSD”. Tsr1ΔNΔloop allowed for generating native crystals that diffracted to 3.6 Å. Here we characterize both wild type Tsr1 and Tsr1ΔNΔloop using cross-linking/mass spectrometry.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Zhuo Chen

LAB HEAD: Juri Rappsilber

PROVIDER: PXD004074 | Pride | 2017-02-08

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Rappsilber_Cook_CLMS_Tsr1_Mutant.raw	Raw
	Rappsilber_Cook_CLMS_Tsr1_Peaklist.zip	Other
	Rappsilber_Cook_CLMS_Tsr1_SearchResult.csv	Csv
	Rappsilber_Cook_CLMS_Tsr1_WildType1.raw	Raw
	Rappsilber_Cook_CLMS_Tsr1_WildType2.raw	Raw

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Publications

FERNANDEZ MARTINEZ ANA ISABEL VILLAVERDE FERNANDEZ MARIO JORGE CASANOVA ROCA JUAN ANTONIO NICOLAS MARTINEZ JOSE ANTONIO MALO LOPEZ-ROMAN JORGE BALLESTA GERMAN CEFERINO

20100331

PMID: ES2378040

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