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Spatiotemporal proteomic profiling of human cerebral development

ABSTRACT: We utilize a rapid shotgun LFQ MS method to profile four regions of developing human cerebrum. Regions encompass neural precursor rich ventricular zone, intermediate, subplate and cortex regions across human development from gestational week 16-36 and postnatal week 41 and adult brain tissue.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: Ugljesa Djuric

LAB HEAD: Eleftherios Diamandis

PROVIDER: PXD004076 | Pride | 2017-07-14

REPOSITORIES: Pride

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			Action	DRS
	108GM1.raw	Raw
	108GM2.raw	Raw
	108IZSP1.raw	Raw
	108IZSP2.raw	Raw
	108SVZ1.raw	Raw

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Publications

Spatiotemporal Proteomic Profiling of Human Cerebral Development.

Djuric Ugljesa U Rodrigues Deivid C DC Batruch Ihor I Ellis James J Shannon Patrick P Diamandis Phedias P

Molecular & cellular proteomics : MCP 20170707 9

Mass spectrometry (MS) analysis of human post-mortem central nervous system (CNS) tissue and induced pluripotent stem cell (iPSC)-based directed differentiations offer complementary avenues to define protein signatures of neurodevelopment. Methodological improvements of formalin-fixed, paraffin-embedded (FFPE) protein isolation now enable widespread proteomic analysis of well-annotated archival tissue samples in the context of development and disease. Here, we utilize a shotgun label-free quanti ...[more]

PMID: 28687556

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Project description:We utilize a rapid shotgun FFPE LFQ MS method to profile four regions of developing human cerebrum. Regions encompass neural precursor rich ventricular zones as well as intermediate and subplate and cortex regions accross human development from gestational weeks 16-36. Proteome signatures of the four fetal brain regions were determined using statistical tests and CMV-infected fetal brain encephalitis was analyzed to determine the region-specific effects of this neurodevelopmental disorder.

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